Linked Life Data

7909332

Source:http://linkedlifedata.com/resource/pubmed/id/7909332

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-5-26
pubmed:abstractText
Complex endocrine relationships exist among the hypothalamus, pituitary, ovaries and thymus. There is also considerable evidence showing gonadotropin releasing hormone (GnRH) involvement in modulating immune system functions. The present study investigated the sequential changes in functional lymphocyte subsets in primary lymphoid tissues of prepubertal female mice in vivo following GnRH agonist treatment in slow release microcapsule formulation. A direct two color immunofluorescence staining followed by flow cytometry was employed. Single i.m injection of agonist significantly decreased both absolute and relative thymic weights and absolute thymocyte counts. No differences, however, were observed in the percentage of thymocytes expressing Thy 1.2, CD4 and CD8. Absolute levels of thymic T cells, CD8 positive cells, immature cells expressing both CD4 and CD8, and immature subsets differentiating toward CD4 were significantly reduced two weeks after agonist treatment. The percentage of bone marrow B cells was also significantly decreased at the second and third weeks following agonist administration. Functional studies to determine in vivo cell-mediated immune function also indicated a significant suppression following agonist administration. These data, together with our earlier observations on secondary lymphoid tissues, suggest a general suppression of lymphocyte maturation at an early stem cell stage of development in prepubertal female mice in vivo.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0165-0378
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
167-84
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Alterations in thymic and bone marrow lymphocyte subpopulations in GnRH agonist treated prepubertal female mice.
pubmed:affiliation
Department of Obstetrics/Gynaecology, MetroHealth Medical Center, Case Western Reserve University, Cleveland, OH.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.