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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1994-5-16
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pubmed:databankReference |
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pubmed:abstractText |
During the adaptation of plants to low temperature, changes in gene expression can be induced in a variety of tissues. Low-temperature-regulated gene expression was studied in cold-stored potato (Solanum tuberosum L.) tubers by two-dimensional electrophoresis of in vitro translation products. As a response to cold treatment, the relative amount of mRNA encoding at least 26 polypeptides changed. By differential screening of a cDNA library, 16 clones corresponding to cold-inducible transcripts were isolated. They were classified into four non-cross-hybridizing groups. RNA hybridizations using representative clones from each group revealed different temporal accumulation patterns for the cold-inducible transcripts. mRNAs homologous to the cDNA clones were first detectable after 1 to 3 d of cold treatment, and the highest level of expression was reached after 3 to 7 d. Transcripts corresponding to cDNA clones CI13 and CI19 were transiently expressed, whereas the steady-state level remained high for cDNA clones CI7 and CI21 during the cold storage period of 4 weeks. The DNA sequences of two cDNA clones, CI7 and CI19, have been determined. The polypeptide predicted from the DNA sequence of CI19 is sequence related to small heat-shock proteins from other plant species. The deduced protein sequence of CI7 exhibits strong homology to the dehydrin/RAB group of dehydration stress- and abscisic acid-inducible polypeptides and to cold-induced proteins from Arabidopsis and spinach.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-1463831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-16453563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-16663813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-16665102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-16665580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-16666159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-16666542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-16667586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-16667647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-1731964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-1830821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-2151719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-2151730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-2263483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-2450022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-2482931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-2562763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-2798141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-2922294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-2970625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-3351943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-4850204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-6329026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909163-8425058
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0032-0889
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
104
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
445-52
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
pubmed-meshheading:7909163-Amino Acid Sequence,
pubmed-meshheading:7909163-Base Sequence,
pubmed-meshheading:7909163-Cloning, Molecular,
pubmed-meshheading:7909163-Cold Temperature,
pubmed-meshheading:7909163-DNA,
pubmed-meshheading:7909163-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:7909163-Gene Expression Regulation,
pubmed-meshheading:7909163-Heat-Shock Proteins,
pubmed-meshheading:7909163-Molecular Sequence Data,
pubmed-meshheading:7909163-Plant Proteins,
pubmed-meshheading:7909163-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:7909163-Protein Biosynthesis,
pubmed-meshheading:7909163-RNA, Messenger,
pubmed-meshheading:7909163-Solanum tuberosum,
pubmed-meshheading:7909163-Transcription, Genetic
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pubmed:year |
1994
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pubmed:articleTitle |
Transcripts accumulating during cold storage of potato (Solanum tuberosum L.) tubers are sequence related to stress-responsive genes.
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pubmed:affiliation |
Max-Planck-Institut für Züchtungsforschung, Köln, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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