Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
1994-4-29
|
| pubmed:abstractText |
In contrast to several inbred strains of mice that develop Th1-associated anticandidal protection, DBA/2 mice are highly susceptible to systemic infection with Candida albicans cells of the attenuated variant PCA-2, and fatal outcome is observed in concurrence with sustained CD4+ cell production in vitro of IL-4 and IL-10. These Th2 cytokines were previously shown to inhibit nitric oxide (NO) production and yeast killing by activated macrophage cultures. We now show that macrophages from DBA/2 mice, either intact or infected with PCA-2, have lower capacity than resistant strains to synthesize NO in response to IFN-gamma. However, when treated with anti-IL-10 Abs at the time of infection, DBA/2 mice survived challenge and displayed increased production of NO in vitro after IFN-gamma activation. Cure was associated with the onset of footpad responses and durable protection, and higher frequencies of IFN-gamma-secreting cells were found in splenic CD4+ lymphocytes that expressed lower levels of IL-4 and IL-10 mRNA. Therefore, in DBA/2 mice, IL-10 contributes significantly to the selection of a Th2 response and lethality after PCA-2 challenge. An IL-10-induced defect in the activation and/or expansion of IFN-gamma-producing Th1 cells, IL-10 suppression of yeast killing, and the relative inability of DBA/2 macrophages to produce adequate levels of candidacidal NO may all contribute to the abnormal susceptibility of these mice to candidiasis.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
152
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3514-21
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:7908304-Animals,
pubmed-meshheading:7908304-Base Sequence,
pubmed-meshheading:7908304-CD4-Positive T-Lymphocytes,
pubmed-meshheading:7908304-Candidiasis,
pubmed-meshheading:7908304-Cytotoxicity, Immunologic,
pubmed-meshheading:7908304-DNA Primers,
pubmed-meshheading:7908304-Female,
pubmed-meshheading:7908304-Gene Expression,
pubmed-meshheading:7908304-Hypersensitivity, Delayed,
pubmed-meshheading:7908304-Immunity, Cellular,
pubmed-meshheading:7908304-Interferon-gamma,
pubmed-meshheading:7908304-Interleukin-10,
pubmed-meshheading:7908304-Interleukin-4,
pubmed-meshheading:7908304-Macrophages,
pubmed-meshheading:7908304-Male,
pubmed-meshheading:7908304-Mice,
pubmed-meshheading:7908304-Mice, Inbred BALB C,
pubmed-meshheading:7908304-Mice, Inbred C57BL,
pubmed-meshheading:7908304-Mice, Inbred DBA,
pubmed-meshheading:7908304-Molecular Sequence Data,
pubmed-meshheading:7908304-Nitric Oxide,
pubmed-meshheading:7908304-RNA, Messenger
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Neutralization of IL-10 up-regulates nitric oxide production and protects susceptible mice from challenge with Candida albicans.
|
| pubmed:affiliation |
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|