Linked Life Data

7907094

Source:http://linkedlifedata.com/resource/pubmed/id/7907094

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1994-4-4
pubmed:abstractText
Deficient expression of glycoinositol phospholipid (GPI)-anchored surface proteins has been linked to six different genetic defects in Thy-1- murine lymphoma mutants. In this study, human K562 cell mutants defective in GPI anchoring were derived by anti-decay-accelerating factor (CD55) based negative fluorescent cell sorting of N-methyl-N'-nitro-N-nitrosoguanidine pretreated cells. Homologous cell fusions of six clones that complemented a previously described K562 mutant corresponding to one of the Thy-1- mutant classes (Hirose, S., Mohney, R. P., Mutka, S. C., Ravi, L., Singleton, D. R., Perry, G., Tartakoff, A., and Medof, M. E. (1992) J. Biol. Chem. 267, 5272-5278) showed that they segregated into two complementation groups. In heterologous cell fusions, representative clones of each group complemented Thy-1 expression by all of the previously described GPI anchor pathway-defective Thy-1- murine lymphoma classes (A, B, C, E, F, and H) but not class(es) D (and I) defective in the Thy-1 structural gene. Analyses of putative GPI anchor precursors synthesized by the two lines revealed that one mutant exhibited a complete block in deacetylation of N-acetyl-D-glucosamine-inositol phospholipid to glucosamine (GlcN)-inositol phospholipid, whereas the other mutant assembled GlcN-inositol phospholipid and subsequent mannose (Man)-containing intermediates but showed markedly increased amounts of the terminal ethanolamine (EthN)-phosphate (P)-substituted putative anchor precursors, EthN-P-6ManMan(EthN-P-->)ManGlcN- and EthN-P-6Man(EthN-P-6)Man(EthN- P-->)ManGlcN-acylinositol phospholipid (H7 and H8). We designate these new complementation classes J, harboring a defect in N-acetyl-D-glucosamine-inositol phospholipid deacetylation, and K, deficient in a step preliminary to or associated with protein transfer of assembled anchor precursors. The availability of these new mutant classes should aid in characterization of the GPI anchor pathway enzymes providing for these reactions.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
269
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6536-42
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:7907094-Animals, pubmed-meshheading:7907094-Antigens, Surface, pubmed-meshheading:7907094-Antigens, Thy-1, pubmed-meshheading:7907094-Carbohydrate Sequence, pubmed-meshheading:7907094-Cell Line, pubmed-meshheading:7907094-Clone Cells, pubmed-meshheading:7907094-Flow Cytometry, pubmed-meshheading:7907094-Glycosylphosphatidylinositols, pubmed-meshheading:7907094-Humans, pubmed-meshheading:7907094-Leukemia, Myelogenous, Chronic, BCR-ABL Positive, pubmed-meshheading:7907094-Lymphoma, pubmed-meshheading:7907094-Membrane Glycoproteins, pubmed-meshheading:7907094-Methylnitronitrosoguanidine, pubmed-meshheading:7907094-Mice, pubmed-meshheading:7907094-Molecular Sequence Data, pubmed-meshheading:7907094-Mutagenesis, pubmed-meshheading:7907094-Phosphatidylinositols, pubmed-meshheading:7907094-Tumor Cells, Cultured, pubmed-meshheading:7907094-Uridine Diphosphate Glucose, pubmed-meshheading:7907094-Uridine Diphosphate N-Acetylglucosamine
pubmed:year
1994
pubmed:articleTitle
Glycoinositol phospholipid anchor-defective K562 mutants with biochemical lesions distinct from those in Thy-1- murine lymphoma mutants.
pubmed:affiliation
Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.