Linked Life Data

7905497

Source:http://linkedlifedata.com/resource/pubmed/id/7905497

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1994-3-9
pubmed:abstractText
Recent studies in the mouse have established that IL-4 and IL-12 direct the development of Ag-stimulated naive CD4 T cells into, respectively, type 2 and type 1 Th cells. We report that prolonged exposure of immunologically naive and unstimulated human neonatal CD4 T cells to IL-4 or to IL-4 plus either IL-2 or IL-12 markedly affects their cytokine production on primary stimulation with PMA and ionomycin. IL-4 induces long-term proliferation of neonatal T cells and after 3 wk of culture these are capable of producing high levels of Th1 (IL-2, IFN-gamma) but no Th2 (IL-4, IL-5) cytokines; IL-4-primed cells are homogenously CD45RO-/RA+ and CD31+. After culture in the presence of IL-4 + IL-2 or IL-4 + IL-12, neonatal T cells are enriched in CD45RO+ and CD31- cells and they can produce Th2 as well as Th1 cytokines. In response to primary stimulation with PMA and ionomycin, cells primed with IL-4 + IL-2 produce IL-4, IL-5, and IL-10 and the same levels of IL-2 and IFN-gamma as IL-4-primed cells. Cells primed with IL-4 + IL-12 produce very high levels of both IL-4 and IFN-gamma but no IL-5. Endogenous IFN-gamma that is detected in primary cultures containing IL-4 + IL-12 does not inhibit, but rather enhances, the ability of the cells to produce IL-4. Further analysis of IL-4 + IL-12-primed cells reveals that IL-4 is mainly produced by CD31- cells and that these cells can trigger B cells to synthesize Ig including IgE. Finally, positively selected CD31+ cells remain CD31+ and are poor IL-4 producers after 3 wk of culture with IL-4 + IL-12, suggesting that these two cytokines promote the selective expansion of the small number of CD31- cells that are present in freshly isolated neonatal CD4 T cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
152
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1141-53
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:7905497-Adult, pubmed-meshheading:7905497-Antigens, CD, pubmed-meshheading:7905497-Antigens, CD31, pubmed-meshheading:7905497-Antigens, Differentiation, Myelomonocytic, pubmed-meshheading:7905497-CD4-CD8 Ratio, pubmed-meshheading:7905497-CD4-Positive T-Lymphocytes, pubmed-meshheading:7905497-Cells, Cultured, pubmed-meshheading:7905497-Humans, pubmed-meshheading:7905497-Immunophenotyping, pubmed-meshheading:7905497-Infant, Newborn, pubmed-meshheading:7905497-Interferon-gamma, pubmed-meshheading:7905497-Interleukin-12, pubmed-meshheading:7905497-Interleukin-2, pubmed-meshheading:7905497-Interleukin-4, pubmed-meshheading:7905497-Interleukins, pubmed-meshheading:7905497-Membrane Glycoproteins, pubmed-meshheading:7905497-T-Lymphocytes, Helper-Inducer, pubmed-meshheading:7905497-Time Factors
pubmed:year
1994
pubmed:articleTitle
In vitro maturation of human neonatal CD4 T lymphocytes. I. Induction of IL-4-producing cells after long-term culture in the presence of IL-4 plus either IL-2 or IL-12.
pubmed:affiliation
University of Montreal, Notre-Dame Hospital Research Center, Canada.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't