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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6A
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pubmed:dateCreated |
1994-2-28
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pubmed:abstractText |
P-glycoprotein (P-gp) detection by FCM (flow cytometry) was correlated with P-gp detection by immunohistochemical staining or Western blotting in K562 cell lines variably resistant to doxorubicin (DOX). A strong correlation was noted between the flow cytometric data and the degree of resistance assessed by thymidine incorporation assay (TIA). Of the human carcinomas tested, 37.5% were P-gp positive by flow cytometry. The flow cytometry data correlated strongly with the immunohistochemical staining (p < 0.001). Thirteen of the 15 P-gp positive tumors were resistant to DOX and vincristine (VCR) on TIA. Thus, P-gp detection by FCM correlates strongly with tumor sensitivity to DOX and VCR (p < 0.001).
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0250-7005
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2171-5
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:7905253-Antibodies, Monoclonal,
pubmed-meshheading:7905253-Carrier Proteins,
pubmed-meshheading:7905253-Cell Division,
pubmed-meshheading:7905253-Doxorubicin,
pubmed-meshheading:7905253-Drug Resistance,
pubmed-meshheading:7905253-Female,
pubmed-meshheading:7905253-Flow Cytometry,
pubmed-meshheading:7905253-Humans,
pubmed-meshheading:7905253-Immunohistochemistry,
pubmed-meshheading:7905253-Leukemia, Myelogenous, Chronic, BCR-ABL Positive,
pubmed-meshheading:7905253-Membrane Glycoproteins,
pubmed-meshheading:7905253-Neoplasms,
pubmed-meshheading:7905253-P-Glycoprotein,
pubmed-meshheading:7905253-Stomach Neoplasms,
pubmed-meshheading:7905253-Thymidine,
pubmed-meshheading:7905253-Tumor Cells, Cultured,
pubmed-meshheading:7905253-Vincristine
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pubmed:articleTitle |
Detection of P-glycoprotein in solid tumors by flow cytometry.
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pubmed:affiliation |
Second Department of Surgery, Fukui Medical School, Japan.
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pubmed:publicationType |
Journal Article
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