Linked Life Data

7904924

Source:http://linkedlifedata.com/resource/pubmed/id/7904924

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-3-3
pubmed:abstractText
1. The leucokinins (LKs) are a group of eight related peptides isolated from the cockroach, Leucophaea maderae. 2. Antisera raised against LK-V, which were specific for the conserved mid to C-terminal region of the LKs, were used to immunostain the parasitic nematode Ascaris suum. 3. LK-IR was observed in neurons in the anterior nerve ring, retrovesicular ganglion, and ventral and dorsal nerve cords of the parasite. Immunostaining was specific in that it was abolished by preabsorption of the antiserum with different leucokinins. Some of the LK-immunoreactive neurons were identified on the basis of their morphological similarity with identified neurons in the free-living nematode Caenorhabditis elegans. 4. Immunoreactivity towards a number of other peptides, notably neuropeptide F, FMRF-amide, KGQELE and KELTAE, has previously been demonstrated in all LK-immunoreactive neurons, thus confirming the multiple-peptidergic nature of certain nematode nerves. 5. LK-IR was demonstrated and quantified in a number of tissues using RIA. Highest amounts were found in extracts of gut; LK-IR was also demonstrated in extracts of body wall, heads and tails, testes, ovaries and pseudocoelomic fluid. 6. The distribution of tissue LK-IR did not correlate with the amount of neuronal tissue in the samples. 7. Dilution experiments suggested that the LK-IR in the parasite is heterogeneous and that the peptide(s) in some tissues may not be analogous to the insect LKs. 8. The LK-IR in the parasite remains to be characterized; however, the results suggest that peptides related to the leucokinins may have a far wider phylogenetic distribution than has hitherto been thought.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0742-8413
pubmed:author
pubmed:issnType
Print
pubmed:volume
106
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
517-22
pubmed:dateRevised
2007-7-23
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Use of specific antisera for the localisation and quantitation of leucokinin immunoreactivity in the nematode, Ascaris suum.
pubmed:affiliation
School of Clinical Medicine, Queen's University of Belfast, Northern Ireland, U.K.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't