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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1994-2-10
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pubmed:abstractText |
Blood contact with synthetic surfaces during cardiopulmonary bypass (CPB) causes a diffuse inflammatory reaction that includes neutrophil activation. The purpose of this study was to determine if inhibition of neutrophil adhesion with a new antiinflammatory agent NPC 15669 (N-(9H-(2,7-dimethylfluorenyl-9-methoxy)-carbonyl)-L-leucine) could reduce pulmonary injury in a porcine model of CPB. NPC 15669 blocks adherence of activated neutrophils by inhibiting upregulation of the Mac-1 (CD11b/CD18) adhesion molecule. Sixteen piglets underwent 2 hours of hypothermic CPB followed by 2 hours of observation; 8 received NPC 15669 (10 mg/kg intravenous bolus followed by 6 mg.kg-1.h-1 intravenous infusion) and 8 received equal volumes of vehicle. After 90 minutes of CPB, expression of neutrophil adhesion molecule subunit CD18 increased 118% in control piglets but only 36% in piglets treated with NPC 15669 (p < 0.01). Although neutropenia developed in all animals during CPB, lung tissue myeloperoxidase content was significantly lower in treated than in control animals 2 hours after CPB (94.9 +/- 10.4 versus 46.9 +/- 5.5 mumol.10 mg-1.min-1; p < 0.002). Free radical-mediated lipid peroxidation (quantitated by spectrophotometric assay of plasma conjugated dienes) was significantly reduced by treatment with NPC 15669 during and after CPB. Pulmonary function was better in NPC 15669-treated animals: 2 hours after CPB, pulmonary vascular resistance increased 477% in control piglets but only 140% in piglets receiving NPC 15669 (p < 0.03); arterial oxygen tension was significantly greater in piglets receiving NPC 15669 (428 +/- 33 mm Hg) than in controls (141 +/- 46; p < 0.0001).(ABSTRACT TRUNCATED AT 250 WORDS)
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Inflammatory Agents...,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD18,
http://linkedlifedata.com/resource/pubmed/chemical/Leucine,
http://linkedlifedata.com/resource/pubmed/chemical/N-(9H-(2,7-dimethylfluoren-9-ylmetho...,
http://linkedlifedata.com/resource/pubmed/chemical/Oxygen
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0003-4975
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
57
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
126-33
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7904144-Animals,
pubmed-meshheading:7904144-Anti-Inflammatory Agents, Non-Steroidal,
pubmed-meshheading:7904144-Antigens, CD,
pubmed-meshheading:7904144-Antigens, CD18,
pubmed-meshheading:7904144-Cell Adhesion,
pubmed-meshheading:7904144-Coronary Artery Bypass,
pubmed-meshheading:7904144-Leucine,
pubmed-meshheading:7904144-Leukocyte Count,
pubmed-meshheading:7904144-Neutrophils,
pubmed-meshheading:7904144-Oxygen,
pubmed-meshheading:7904144-Swine,
pubmed-meshheading:7904144-Vascular Resistance
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pubmed:year |
1994
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pubmed:articleTitle |
Inhibition of neutrophil adhesion during cardiopulmonary bypass.
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pubmed:affiliation |
Department of Cardiac Surgery, Johns Hopkins Medical Institutions, Baltimore, Maryland.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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