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rdf:type |
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lifeskim:mentions |
umls-concept:C0014442,
umls-concept:C0014834,
umls-concept:C0017366,
umls-concept:C0020205,
umls-concept:C0032136,
umls-concept:C0034865,
umls-concept:C0059944,
umls-concept:C0206243,
umls-concept:C0441513,
umls-concept:C1314939,
umls-concept:C1521871,
umls-concept:C1527177
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pubmed:issue |
10
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pubmed:dateCreated |
1976-12-30
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pubmed:abstractText |
Endo-R-HindIII restriction endonuclease fragments obtained from F30 and pMB9 plasmid DNAs were ligated in vitro and used to transform a recB21 recC22 sbcB15 strain of E. coli K-12. The inability of this strain to stably maintain pMB9 alone permitted the isolation of transformants that carried hybrid plasmids containing the sbcB+ allele. These transformants became sensitive to ultraviolet light and recombination defieient and showed a 25-fold increase in the level of exonuclease I activity. The stability of the sbcB hybrid plasmids and their effects on exonuclease I activity have also been determined in wild-type and recA1 genetic backgrounds. The presence of the plasmids results in a 7-fold increase in the level of exonuclease I in a wild-type strain and 15-fold increase in a recA1 strain. The increased activity in the recA1 mutant appears to be a result of increased plasmid stability in this genetic background.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-1090574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-1103146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-1105581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-1267736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-14114849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-14114850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-14114851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-14235546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-14294081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4205905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4336040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4372397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4552016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4552924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4554537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4559594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4562227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4567284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4587405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4588280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4595200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4602029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4607228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4610576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4610579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4612007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4612008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4630612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4879167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4884815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4894835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4901366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4902143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4924002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4927675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-4965137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-5961488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-767321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/790387-773363
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
73
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3492-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:790387-Base Sequence,
pubmed-meshheading:790387-DNA, Bacterial,
pubmed-meshheading:790387-DNA, Recombinant,
pubmed-meshheading:790387-Escherichia coli,
pubmed-meshheading:790387-Exonucleases,
pubmed-meshheading:790387-Extrachromosomal Inheritance,
pubmed-meshheading:790387-Genes,
pubmed-meshheading:790387-Genetic Engineering,
pubmed-meshheading:790387-Genotype,
pubmed-meshheading:790387-Plasmids,
pubmed-meshheading:790387-Recombination, Genetic
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pubmed:year |
1976
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pubmed:articleTitle |
Amplification in Escherichia coli of enzymes involved in genetic recombination: construction of hybrid ColE1 plasmids carrying the structural gene for exonuclease I.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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