Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1994-1-31
|
| pubmed:abstractText |
A mutation in the alpha 1-tubulin gene of Chlamydomonas reinhardtii was isolated by using the amiprophos-methyl-resistant mutation apm1-18 as a background to select new mutants that showed increased resistance to the drug. The upA12 mutation caused twofold resistance to amiprophos-methyl and oryzalin, and twofold hypersensitivity to the microtubule-stabilizing drug taxol, suggesting that the mutation enhanced microtubule stability. The resistance mutation was semi-dominant and mapped to the same interval on linkage group III as the alpha 1-tubulin gene. Two-dimensional gel immunoblots of proteins in the mutant cells revealed two electrophoretically altered alpha-tubulin isoforms, one of which was acetylated and incorporated into microtubules in the axoneme. The mutant isoforms co-segregated with the drug-resistance phenotypes when mutant upA12 was backcrossed to wild-type cells. Two-dimensional gel analysis of in vitro translation products showed that the non-acetylated variant alpha-tubulin was a primary gene product. DNA sequence analysis of the alpha 1-tubulin genes from mutant and wild-type cells revealed a single missense mutation, which predicted a change in codon 24 from tyrosine in wild type to histidine in mutant upA12. This alteration in the predicted amino acid sequence corroborated the approximately +1 basic charge shift observed for the variant alpha-tubulins. The mutant allele of the alpha 1-tubulin gene was designated tua1-1.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Dinitrobenzenes,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrobenzenes,
http://linkedlifedata.com/resource/pubmed/chemical/Organothiophosphorus Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Paclitaxel,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protozoan Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfanilamides,
http://linkedlifedata.com/resource/pubmed/chemical/Tubulin,
http://linkedlifedata.com/resource/pubmed/chemical/amiprophos,
http://linkedlifedata.com/resource/pubmed/chemical/oryzalin
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0021-9533
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
106 ( Pt 1)
|
| pubmed:geneSymbol |
ac17,
apm1-18,
nit2,
ory1-2,
pdr1-1,
pf5,
tua1-1,
upA12
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
209-18
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7903670-Acetylation,
pubmed-meshheading:7903670-Alleles,
pubmed-meshheading:7903670-Animals,
pubmed-meshheading:7903670-Chlamydomonas reinhardtii,
pubmed-meshheading:7903670-DNA Mutational Analysis,
pubmed-meshheading:7903670-Dinitrobenzenes,
pubmed-meshheading:7903670-Drug Resistance,
pubmed-meshheading:7903670-Genes, Plant,
pubmed-meshheading:7903670-Genes, Protozoan,
pubmed-meshheading:7903670-Microtubules,
pubmed-meshheading:7903670-Nitrobenzenes,
pubmed-meshheading:7903670-Organothiophosphorus Compounds,
pubmed-meshheading:7903670-Paclitaxel,
pubmed-meshheading:7903670-Plant Proteins,
pubmed-meshheading:7903670-Protein Processing, Post-Translational,
pubmed-meshheading:7903670-Protozoan Proteins,
pubmed-meshheading:7903670-Sulfanilamides,
pubmed-meshheading:7903670-Tubulin
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
A mutation in the alpha 1-tubulin gene of Chlamydomonas reinhardtii confers resistance to anti-microtubule herbicides.
|
| pubmed:affiliation |
Department of Genetics and Cell Biology, University of Minnesota, St. Paul 55108-1095.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|