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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1994-1-21
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pubmed:abstractText |
Amplification and overexpression of the neu (c-erbB2) proto-oncogene has been implicated in the pathogenesis of 20 to 30% of human breast cancers. Although the activation of Neu receptor tyrosine kinase appears to be a pivotal step during mammary tumorigenesis, the mechanism by which Neu signals cell proliferation is unclear. Molecules bearing a domain shared by the c-Src proto-oncogene (Src homology 2) are thought to be involved in signal transduction from activated receptor tyrosine kinases such as Neu. To test whether c-Src was implicated in Neu-mediated signal transduction, we measured the activity of the c-Src tyrosine kinase in tissue extracts from either mammary tumors or adjacent mammary epithelium derived from transgenic mice expressing a mouse mammary tumor virus promoter/enhancer/unactivated neu fusion gene. The Neu-induced mammary tumors possessed six- to eightfold-higher c-Src kinase activity than the adjacent epithelium. The increase in c-Src tyrosine kinase activity was not due to an increase in the levels of c-Src but rather was a result of the elevation of its specific activity. Moreover, activation of c-Src was correlated with its ability to complex tyrosine-phosphorylated Neu both in vitro and in vivo. Together, these observations suggest that activation of the c-Src tyrosine kinase during mammary tumorigenesis may occur through a direct interaction with activated Neu.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-1312220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-1359541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-1380891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-1408131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-1545818,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-1670762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-1672253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-1672440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-1683701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-1696179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-1904008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-1971420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-1997203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-2470152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-2537153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-2871941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-2885917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-2890160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-2898299,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-2992089,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-7681217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-7681396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-8356071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7903421-8394206
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:geneSymbol |
neu
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
735-43
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pubmed:dateRevised |
2011-11-2
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pubmed:meshHeading |
pubmed-meshheading:7903421-Animals,
pubmed-meshheading:7903421-Breast Neoplasms,
pubmed-meshheading:7903421-Enzyme Activation,
pubmed-meshheading:7903421-Female,
pubmed-meshheading:7903421-Gene Expression,
pubmed-meshheading:7903421-Genes, myc,
pubmed-meshheading:7903421-Humans,
pubmed-meshheading:7903421-Mammary Neoplasms, Experimental,
pubmed-meshheading:7903421-Mammary Tumor Virus, Mouse,
pubmed-meshheading:7903421-Mice,
pubmed-meshheading:7903421-Mice, Transgenic,
pubmed-meshheading:7903421-Protein-Tyrosine Kinases,
pubmed-meshheading:7903421-Proto-Oncogene Proteins,
pubmed-meshheading:7903421-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:7903421-Proto-Oncogenes,
pubmed-meshheading:7903421-Receptor, Epidermal Growth Factor,
pubmed-meshheading:7903421-Receptor, erbB-2,
pubmed-meshheading:7903421-Signal Transduction
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pubmed:year |
1994
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pubmed:articleTitle |
Mammary tumors expressing the neu proto-oncogene possess elevated c-Src tyrosine kinase activity.
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pubmed:affiliation |
Institute for Molecular Biology and Biotechnology, McMaster University, Hamilton, Ontario, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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