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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1994-1-21
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pubmed:abstractText |
The Polycomb group (Pc-G) proteins are responsible for keeping developmental regulators, like homeotic genes, stably and inheritably repressed during Drosophila development. Several similarities to a protein class involved in heterochromatin formation suggest that the Pc-G exerts its function at the higher order chromatin level. Here we have mapped the distribution of the Pc protein in the homeotic bithorax complex (BX-C) of Drosophila tissue culture cells. We have elaborated a method, based on the in vivo formaldehyde cross-linking technique, that allows a substantial enrichment for Pc-interacting sites by immunoprecipitation of the cross-linked chromatin with anti-Pc antibodies. We find that the Pc protein quantitatively covers large regulatory regions of repressed BX-C genes. Conversely, we find that the Abdominal-B gene is active in these cells and the region devoid of any bound Pc protein.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chromatin,
http://linkedlifedata.com/resource/pubmed/chemical/Cross-Linking Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Formaldehyde,
http://linkedlifedata.com/resource/pubmed/chemical/Heterochromatin,
http://linkedlifedata.com/resource/pubmed/chemical/Insect Hormones,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Polycomb protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1187-98
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7903220-Animals,
pubmed-meshheading:7903220-Base Sequence,
pubmed-meshheading:7903220-Binding Sites,
pubmed-meshheading:7903220-Blotting, Southern,
pubmed-meshheading:7903220-Cells, Cultured,
pubmed-meshheading:7903220-Chromatin,
pubmed-meshheading:7903220-Cloning, Molecular,
pubmed-meshheading:7903220-Cross-Linking Reagents,
pubmed-meshheading:7903220-DNA,
pubmed-meshheading:7903220-Drosophila Proteins,
pubmed-meshheading:7903220-Drosophila melanogaster,
pubmed-meshheading:7903220-Formaldehyde,
pubmed-meshheading:7903220-Genes, Homeobox,
pubmed-meshheading:7903220-Heterochromatin,
pubmed-meshheading:7903220-Insect Hormones,
pubmed-meshheading:7903220-Molecular Sequence Data,
pubmed-meshheading:7903220-Oligodeoxyribonucleotides,
pubmed-meshheading:7903220-Polymerase Chain Reaction,
pubmed-meshheading:7903220-Proteins,
pubmed-meshheading:7903220-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:7903220-Restriction Mapping
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pubmed:year |
1993
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pubmed:articleTitle |
Mapping Polycomb-repressed domains in the bithorax complex using in vivo formaldehyde cross-linked chromatin.
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pubmed:affiliation |
Zentrum für Molekulare Biologie, Universität Heidelberg, Federal Republic of Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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