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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1994-1-6
|
| pubmed:abstractText |
Seventeen strains of Escherichia coli serotype O157:H7 producing Shiga-like toxin were examined for the presence of plasmids and for the ability to adhere to HEp-2 and Intestine 407 cells. All of the strains possessed a common 60-MDa plasmid. To determine the role of the 60-MDa plasmid, plasmid-cured strains were compared with the parent strains for their ability to produce pili and to adhere to epithelial cells in culture. The total cell lysate protein and outer-membrane protein (OMP) profiles were also compared. Both the parent strains and their plasmid-cured derivatives produced pili. Immunofluorescence assay results indicated that the plasmid-cured and parent strains adhered equally well to HEp-2 and Intestine 407 cells; overall adherence was greater with intestinal cells than HEp-2 cells. SDS-PAGE of polypeptides synthesised in an E. coli system in vitro showed that plasmid DNA encodes c. 35 proteins. SDS-PAGE of OMP preparations demonstrated that the 60-MDa plasmid appears to be involved in the synthesis of a 33-kDa OMP. Two strains cured of the 60-MDa plasmid, one that possessed no plasmids and one that still contained a 2.2-MDa plasmid, produced exopolysaccharide (EPS) when cultured on solid medium at 25 degrees C. Two other strains, which were cured of the 60-MDa plasmid but contained a 4.5-MDa plasmid, did not produce visible amounts of EPS. Gas chromatography analysis showed that the EPS consisted of fucose, glucose and galactose in an approximate molar ratio of 2.0:0.9:1.1 and also had 7% of a uronic acid sugar as part of its structure.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Outer Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Toxins,
http://linkedlifedata.com/resource/pubmed/chemical/Cytotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Polysaccharides, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Shiga Toxin 1
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0022-2615
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
39
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
371-81
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7902442-Bacterial Adhesion,
pubmed-meshheading:7902442-Bacterial Outer Membrane Proteins,
pubmed-meshheading:7902442-Bacterial Proteins,
pubmed-meshheading:7902442-Bacterial Toxins,
pubmed-meshheading:7902442-Chromatography, Gas,
pubmed-meshheading:7902442-Colitis,
pubmed-meshheading:7902442-Cytotoxins,
pubmed-meshheading:7902442-DNA, Bacterial,
pubmed-meshheading:7902442-Escherichia coli,
pubmed-meshheading:7902442-Escherichia coli Infections,
pubmed-meshheading:7902442-Fimbriae, Bacterial,
pubmed-meshheading:7902442-Fluorescent Antibody Technique,
pubmed-meshheading:7902442-Food Microbiology,
pubmed-meshheading:7902442-Gastrointestinal Hemorrhage,
pubmed-meshheading:7902442-Humans,
pubmed-meshheading:7902442-Microscopy, Electron, Scanning,
pubmed-meshheading:7902442-Plasmids,
pubmed-meshheading:7902442-Polysaccharides, Bacterial,
pubmed-meshheading:7902442-Shiga Toxin 1,
pubmed-meshheading:7902442-Virulence
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Studies on Escherichia coli serotype O157:H7 strains containing a 60-MDa plasmid and on 60-MDa plasmid-cured derivatives.
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| pubmed:affiliation |
Microbial Food Safety Research Unit, US Department of Agriculture, Philadelphia, PA 19118.
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| pubmed:publicationType |
Journal Article
|