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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1993-12-9
|
| pubmed:abstractText |
In the present study we characterized the frequencies of two polymorphisms within the MHC-linked heat shock protein (HSP) 70 genes in patients with insulin-dependent diabetes mellitus (IDDM) (n = 114) and healthy control individuals (n = 110). Significant differences in genotype and allelic frequencies were observed for both polymorphisms between randomly selected patients and controls. However, for the HSP70-2 polymorphisms this was solely due to linkage disequilibrium with DR3. The rate HSP70-Hom 2-allele was significantly more frequent in controls than in patients. It showed strong association with certain tumour necrosis factor (TNF) (class III) and HLA-B and -A (class I) alleles independent of HLA-DQ and -DR alleles. By typing 257 individuals from 55 IDDM multiple-case families two extended MHC-haplotypes, including class II-, TNF- and class I-markers, carrying the rare HSP70-Hom allele were defined. One was only transmitted to diabetic offspring, whereas the other was only transmitted to unaffected offspring. The functional implication of the polymorphism in the heat shock-inducible HSP70-2 gene was analysed by studying HSP70-2 mRNA expression after heat shock in peripheral blood mononuclear cells from individuals with different HSP70-2 genotypes. Preliminary data showed that individuals homozygous for the PstI 8.5-kb allele consistently had slightly lower expression than heterozygous and 9.0-kb homozygous individuals.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0300-9475
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
38
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
491-5
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:7901896-Adult,
pubmed-meshheading:7901896-Alleles,
pubmed-meshheading:7901896-Base Sequence,
pubmed-meshheading:7901896-Diabetes Mellitus, Type 1,
pubmed-meshheading:7901896-Female,
pubmed-meshheading:7901896-Gene Expression,
pubmed-meshheading:7901896-Gene Frequency,
pubmed-meshheading:7901896-Genotype,
pubmed-meshheading:7901896-Heat-Shock Proteins,
pubmed-meshheading:7901896-Histocompatibility Testing,
pubmed-meshheading:7901896-Humans,
pubmed-meshheading:7901896-Major Histocompatibility Complex,
pubmed-meshheading:7901896-Male,
pubmed-meshheading:7901896-Molecular Sequence Data,
pubmed-meshheading:7901896-Polymorphism, Genetic,
pubmed-meshheading:7901896-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:7901896-RNA, Messenger,
pubmed-meshheading:7901896-Random Allocation
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Polymorphic analysis of the human MHC-linked heat shock protein 70 (HSP70-2) and HSP70-Hom genes in insulin-dependent diabetes mellitus (IDDM).
|
| pubmed:affiliation |
Steno Diabetes Center, Gentofte, Denmark.
|
| pubmed:publicationType |
Journal Article
|