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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1993-11-29
|
| pubmed:abstractText |
Glutamic acid and glycine were quantified in cells and medium of cultured rostral rhombencephalic neurons derived from fetal rats. In the presence of 1 mM Mg2+, NMDA (50 microM) significantly stimulated (by 69%) release of newly synthesized 5-[3H]hydroxytryptamine ([3H]5-HT). D-2-Amino-5-phosphonopentanoate (AP-5; 50 microM) blocked the stimulatory effect of NMDA. AP-5 by itself inhibited [3H]5-HT release (by 25%), suggesting a tonic control of 5-HT by glutamate. In the absence of Mg2+, basal [3H]5-HT release was 60% higher as compared with release with Mg2+. AP-5 blocked the increased [3H]5-HT release observed without Mg2+, suggesting that this effect was due to the stimulation of NMDA receptors by endogenous glutamate. Glycine (100 microM) inhibited [3H]5-HT release in the absence of Mg2+. Strychnine (50 microM) blocked the inhibitory effect of glycine, indicating an action through strychnine-sensitive inhibitory glycine receptors. The [3H]5-HT release stimulated by NMDA was unaffected by glycine. In contrast, when tested in the presence of strychnine, glycine increased NMDA-evoked [3H]5-HT release (by 22%), and this effect was prevented by a selective antagonist of the NMDA-associated glycine receptor, 7-chlorokynurenate (100 microM). 7-Chlorokynurenate by itself induced a drastic decrease in [3H]5-HT release, indicating that under basal conditions these sites were stimulated by endogenous glycine. These results indicate that NMDA stimulated [3H]5-HT release in both the presence or absence of Mg2+. Use of selective antagonists allowed differentiation of a strychnine-sensitive glycine response (inhibition of [3H]5-HT release) from a 7-chlorokynurenate-sensitive response (potentiation of NMDA-evoked [3H]5-HT release).
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-amino-5-phosphopentanoic acid,
http://linkedlifedata.com/resource/pubmed/chemical/7-chlorokynurenic acid,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamates,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Glycine,
http://linkedlifedata.com/resource/pubmed/chemical/Kynurenic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/N-Methylaspartate,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Glycine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, N-Methyl-D-Aspartate,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Strychnine,
http://linkedlifedata.com/resource/pubmed/chemical/Valine
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0022-3042
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
61
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1692-7
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7901329-Animals,
pubmed-meshheading:7901329-Cells, Cultured,
pubmed-meshheading:7901329-Embryo, Mammalian,
pubmed-meshheading:7901329-Female,
pubmed-meshheading:7901329-Gestational Age,
pubmed-meshheading:7901329-Glutamates,
pubmed-meshheading:7901329-Glutamic Acid,
pubmed-meshheading:7901329-Glycine,
pubmed-meshheading:7901329-Kinetics,
pubmed-meshheading:7901329-Kynurenic Acid,
pubmed-meshheading:7901329-N-Methylaspartate,
pubmed-meshheading:7901329-Pregnancy,
pubmed-meshheading:7901329-Raphe Nuclei,
pubmed-meshheading:7901329-Rats,
pubmed-meshheading:7901329-Rats, Sprague-Dawley,
pubmed-meshheading:7901329-Receptors, Glycine,
pubmed-meshheading:7901329-Receptors, N-Methyl-D-Aspartate,
pubmed-meshheading:7901329-Rhombencephalon,
pubmed-meshheading:7901329-Serotonin,
pubmed-meshheading:7901329-Strychnine,
pubmed-meshheading:7901329-Valine
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
N-methyl-D-aspartic acid/glycine interactions on the control of 5-hydroxytryptamine release in raphe primary cultures.
|
| pubmed:affiliation |
Laboratoire de Neuroendocrinologie Expérimentale, INSERM U. 297, UER Faculté de Médecine-Nord, Marseille, France.
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| pubmed:publicationType |
Journal Article
|