Linked Life Data

7901228

Source:http://linkedlifedata.com/resource/pubmed/id/7901228

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1993-12-3
pubmed:abstractText
The murine Hoxa-4 gene encodes a protein with a homeodomain closely related to those produced by the Antennapedia-like class of Drosophila genes. Drosophila homeodomain proteins can function as transcription factors, binding to several specific DNA sequences. One sequence that is frequently encountered contains a core ATTA motif within a larger consensus sequence, such as CAATTAA. The in vitro synthesized protein product of Hoxa-4 was shown to bind to a subset of restriction fragments of the Hoxa-4 gene itself as determined by gel retardation experiments. Direct examination of the sequences of the fragments bound by Hoxa-4 protein revealed the presence of four regions containing the core ATTA motif. Two regions contained sequences of the CAATTAA class and were located approximately 1 kb upstream from the putative somatic Hoxa-4 promoter and within the intron. Two additional binding sites containing the consensus target sequence involved in autoregulation of Drosophila Deformed gene were identified: one immediately downstream of the putative embryonic transcription start site and one within the intron, respectively. Specific binding of the in vitro produced Hoxa-4 protein to oligonucleotides corresponding to these sequences was observed in gel retardation assays. The same results were obtained with Hoxa-4 protein produced in a Baculovirus expression system. Experiments using oligonucleotides containing base substitutions in positions 1, 3, 4, and 5 in the sequence CAATTAA showed severely reduced binding. The use of truncated mutant Hoxa-4 proteins in gel retardation assays and in transient co-transfection experiments revealed that the intact homeodomain was required for the binding. These results also suggested that the Hoxa-4 gene has the potential to auto-regulate its expression by interacting with the homeodomain binding sites present in the promoter as well as in the intron.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0730-2312
pubmed:author
pubmed:issnType
Print
pubmed:volume
52
pubmed:geneSymbol
Dfd, HOX3D, HOX4C, HOX4D, HOX6A, Hox-1.4, Hox-2.2, Hox-4.2, Hox-C, Hoxa-4, Hoxb-4, Hoxb-6, Hoxd-4, ftz
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
449-62
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:7901228-Amino Acid Sequence, pubmed-meshheading:7901228-Animals, pubmed-meshheading:7901228-Base Sequence, pubmed-meshheading:7901228-Binding Sites, pubmed-meshheading:7901228-Consensus Sequence, pubmed-meshheading:7901228-DNA, pubmed-meshheading:7901228-DNA-Binding Proteins, pubmed-meshheading:7901228-Fushi Tarazu Transcription Factors, pubmed-meshheading:7901228-Genes, Homeobox, pubmed-meshheading:7901228-Homeodomain Proteins, pubmed-meshheading:7901228-Insect Hormones, pubmed-meshheading:7901228-Introns, pubmed-meshheading:7901228-Mice, pubmed-meshheading:7901228-Molecular Sequence Data, pubmed-meshheading:7901228-Promoter Regions, Genetic, pubmed-meshheading:7901228-Protein Binding, pubmed-meshheading:7901228-Recombinant Fusion Proteins, pubmed-meshheading:7901228-Transcription, Genetic
pubmed:year
1993
pubmed:articleTitle
Protein product of the somatic-type transcript of the Hoxa-4 (Hox-1.4) gene binds to homeobox consensus binding sites in its promoter and intron.
pubmed:affiliation
Department of Biochemistry and Molecular Biophysics, Columbia University College of Physicians and Surgeons, New York, New York 10032.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't