|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0019564,
umls-concept:C0028351,
umls-concept:C0030685,
umls-concept:C0032433,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0039067,
umls-concept:C0079883,
umls-concept:C0391871,
umls-concept:C0680255,
umls-concept:C0851285,
umls-concept:C1283071,
umls-concept:C1963578,
umls-concept:C2263130
|
|
pubmed:issue |
11
|
|
pubmed:dateCreated |
1995-4-26
|
|
pubmed:abstractText |
The role of polyamines (PA) synthesis in NMDA receptor-mediated 45Ca2+ fluxes and norepinephrine release was studied in rat hippocampal synaptosomes. NMDA (50 microM) caused a sharp (> 2-fold) transient increase in PA synthesis regulating enzyme, ornithine decarboxylase (ODC) activity with concomitant elevation in PA levels in the order putrescine > spermidine > spermine. ODC inhibitor, alpha-difluoromethylornithine (DFMO), and NMDA antagonist, 2-amino-5-phosphonovaleric acid (D-AP5), both blocked increases in ODC activity and PA levels. Activation of NMDA receptors induced a sharp (3 to 4-fold) and quick (15 seconds) increase in 45Ca2+ uptake by synaptosomes within 15 seconds of exposure at 37 degrees C. The efflux of 45Ca2+ and 3H-norepinephrine (NE) release at 22 degrees C from pre-loaded synaptosomes was also significantly (2 to 4-fold) enhanced by NMDA within 15 seconds. These NMDA receptor-mediated effects on calcium fluxes and NE release were blocked by NMDA receptor-antagonists (DAP-5 and MK-801) and PA synthesis inhibitor, DFMO and the DFMO inhibition nullified by exogenous putrescine. These observations establish that ODC/PA cascade play an important role in transduction of excitatory amino acid mediated signals at NMDA receptors.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-Amino-5-phosphonovalerate,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Eflornithine,
http://linkedlifedata.com/resource/pubmed/chemical/Egtazic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/N-Methylaspartate,
http://linkedlifedata.com/resource/pubmed/chemical/Norepinephrine,
http://linkedlifedata.com/resource/pubmed/chemical/Ornithine Decarboxylase,
http://linkedlifedata.com/resource/pubmed/chemical/Polyamines,
http://linkedlifedata.com/resource/pubmed/chemical/Putrescine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, N-Methyl-D-Aspartate,
http://linkedlifedata.com/resource/pubmed/chemical/Spermidine,
http://linkedlifedata.com/resource/pubmed/chemical/Spermine
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0364-3190
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
19
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1421-9
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:7898610-2-Amino-5-phosphonovalerate,
pubmed-meshheading:7898610-Animals,
pubmed-meshheading:7898610-Biological Transport,
pubmed-meshheading:7898610-Calcium,
pubmed-meshheading:7898610-Eflornithine,
pubmed-meshheading:7898610-Egtazic Acid,
pubmed-meshheading:7898610-Female,
pubmed-meshheading:7898610-Hippocampus,
pubmed-meshheading:7898610-Kinetics,
pubmed-meshheading:7898610-N-Methylaspartate,
pubmed-meshheading:7898610-Norepinephrine,
pubmed-meshheading:7898610-Ornithine Decarboxylase,
pubmed-meshheading:7898610-Polyamines,
pubmed-meshheading:7898610-Putrescine,
pubmed-meshheading:7898610-Rats,
pubmed-meshheading:7898610-Rats, Sprague-Dawley,
pubmed-meshheading:7898610-Receptors, N-Methyl-D-Aspartate,
pubmed-meshheading:7898610-Spermidine,
pubmed-meshheading:7898610-Spermine,
pubmed-meshheading:7898610-Synaptosomes
|
|
pubmed:year |
1994
|
|
pubmed:articleTitle |
Regulation of N-methyl-D-aspartate receptor-mediated calcium transport and norepinephrine release in rat hippocampus synaptosomes by polyamines.
|
|
pubmed:affiliation |
Department of Neurology, Northwestern University Medical School, Illinois 60611-3008.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|
