Linked Life Data

7895840

Source:http://linkedlifedata.com/resource/pubmed/id/7895840

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-4-21
pubmed:abstractText
A murine model system was developed to study the induction and mechanism of protective immunity to L3 of Strongyloides stercoralis. L3 were implanted in BALB/cByJ mice in diffusion chambers constructed with 0.1- or 2.0-microns-pore-size membranes. Parasites survived equally well regardless or membrane type for 7 days, after which larval survival decreased in diffusion chambers constructed with 2.0-microns-pore-size membranes, which allowed host cells to enter. Survival of S. stercoralis L3 in diffusion chambers implanted in mice was assayed after immunization with live, heat-killed, and homogenized L3. Optimal immunization was achieved with 10,000 live L3, whereby immunized mice eliminated 97% of the larvae either contained within diffusion chambers or free within the tissues of the mouse by 24 hr postinfection. Sera from immunized mice had elevated levels of IgG1, IgM, and IgA parasitic-specific antibody; IgM was the only antibody isotype that recognized surface antigens of L3. Larvae were not killed in immunized mice if contact between host cells and the parasites was prevented. In the peripheral blood and diffusion chamber fluid of immunized mice, eosinophil levels were significantly higher when compared to the levels found in control mice. The rodent model developed in the present study has thus demonstrated that virtually complete immunity can be induced to the L3 of S. stercoralis and that larval killing was found to be associated with the presence of both specific antibody and eosinophils.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0014-4894
pubmed:author
pubmed:issnType
Print
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
297-307
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Strongyloides stercoralis: protective immunity to third-stage larvae inBALB/cByJ mice.
pubmed:affiliation
Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't