Linked Life Data

7894159

Source:http://linkedlifedata.com/resource/pubmed/id/7894159

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1995-4-27
pubmed:abstractText
By fusion of thymidine kinase-deficient mink cells with pig leukocytes, a new type of cell hybrid was produced. It was demonstrated that pig chromosomes segregate in pig-mink hybrids and that hybrid cells contain no cytologically visible rearrangements between the chromosomes of parental species, or chromosome fragmentation. With a set of subclones of two primary hybrid clones, the genes for thymidine kinase-1 (TK1) and uridine 5'-monophosphate hydrolase-2 (UMPH2) were assigned to pig Chromosome (Chr) 12. A cell line with a single pig Chr 8 on the background of mink chromosomes was established. This clone could serve as a source of DNA for building a chromosome-specific library of pig Chr 8. The data obtained suggest that pig-mink cell hybrids can be used for mapping of pig chromosomes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0938-8990
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:geneSymbol
TK1, UMPH2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
781-4
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Characterization of pig-mink cell hybrids: assignment of the TK1 and UMPH2 genes to pig chromosome 12.
pubmed:affiliation
Developmental Genetics Laboratory, Russian Academy of Sciences, Siberian Department, Novosibirsk.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't