rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5205
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pubmed:dateCreated |
1995-4-14
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pubmed:abstractText |
Nae I endonuclease must bind to two DNA sequences for cleavage. Examination of the amino acid sequence of Nae I uncovered similarity to the active site of human DNA ligase I, except for leucine 43 in Nae I instead of the lysine essential for ligase activity. Changing leucine 43 to lysine 43 (L43K) changed Nae I activity: Nae I-L43K relaxed supercoiled DNA to yield DNA topoisomers and recombined DNA to give dimeric molecules. Interruption of the reactions of Nae I and Nae I-L43K with DNA demonstrated transient protein-DNA covalent complexes. These findings imply coupled endonuclease and ligase domains and link Nae I endonuclease to the topoisomerase and recombinase protein families.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Circular,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Nucleotidyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Topoisomerases, Type I,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonucleases, Type II...,
http://linkedlifedata.com/resource/pubmed/chemical/Integrases,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinases,
http://linkedlifedata.com/resource/pubmed/chemical/endodeoxyribonuclease NaeI,
http://linkedlifedata.com/resource/pubmed/chemical/integron integrase IntI1
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
24
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pubmed:volume |
267
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1817-20
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pubmed:dateRevised |
2007-3-19
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pubmed:meshHeading |
pubmed-meshheading:7892605-Amino Acid Sequence,
pubmed-meshheading:7892605-Binding Sites,
pubmed-meshheading:7892605-DNA, Circular,
pubmed-meshheading:7892605-DNA Nucleotidyltransferases,
pubmed-meshheading:7892605-DNA Topoisomerases, Type I,
pubmed-meshheading:7892605-Deoxyribonucleases, Type II Site-Specific,
pubmed-meshheading:7892605-Integrases,
pubmed-meshheading:7892605-Molecular Sequence Data,
pubmed-meshheading:7892605-Mutagenesis, Site-Directed,
pubmed-meshheading:7892605-Recombinases,
pubmed-meshheading:7892605-Sequence Homology, Amino Acid
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pubmed:year |
1995
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pubmed:articleTitle |
DNA topoisomerase and recombinase activities in Nae I restriction endonuclease.
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pubmed:affiliation |
Lineberger Comprehensive Cancer Center, Department of Biochemistry and Biophysics, University of North Carolina Medical School, Chapel Hill 27599.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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