Linked Life Data

7891660

Source:http://linkedlifedata.com/resource/pubmed/id/7891660

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1995-4-14
pubmed:databankReference
pubmed:abstractText
A beta-glucosidase/xylosidase gene from Erwinia chrysanthemi strain D1 was cloned and sequenced. This gene, named bgxA, encodes a ca. 71 kDa protein product which, following removal of the leader peptide, resulted in a ca. 69 kDa mature protein that accumulated in the periplasmic space of E. chrysanthemi strain D1 and Escherichia coli cells expressing the cloned gene. The protein exhibited both beta-glucosidase and beta-xylosidase activities but gave no detectable activity on xylan or carboxymethyl cellulose. The enzyme was classified as a type 3 glycosyl hydrolase, but was unusual in having a truncated B region at the carboxyl-terminus. Several E. chrysanthemi strains isolated from corn produced the glucosidase/xylosidase activity but not those isolated from dicot plants. However, bgxA marker exchange mutants of strain D1 were not detectably altered in virulence on corn leaves.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0026-8925
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
246
pubmed:geneSymbol
bgxA
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
465-77
pubmed:dateRevised
2006-5-1
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Cloning and characterization of the bgxA gene from Erwinia chrysanthemi D1 which encodes a beta-glucosidase/xylosidase enzyme.
pubmed:affiliation
Department of Plant Pathology, University of California, Riverside 92521.
pubmed:publicationType
Journal Article