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rdf:type |
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lifeskim:mentions |
umls-concept:C0030685,
umls-concept:C0127400,
umls-concept:C0178719,
umls-concept:C0205314,
umls-concept:C0225336,
umls-concept:C0391871,
umls-concept:C0439799,
umls-concept:C0596235,
umls-concept:C0679622,
umls-concept:C0680255,
umls-concept:C1283071,
umls-concept:C1698986,
umls-concept:C1963578
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pubmed:issue |
10
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pubmed:dateCreated |
1995-4-14
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pubmed:abstractText |
Sphingolipid-gated Ca2+ signaling is mediated through Ca(2+)-permeable channels. In this report, we characterize the properties of the channel in a human endothelial cell line (EA.hy926). Ca2+ release from intracellular stores is not antagonized by nifedipine, omega conotoxin G-VIa, or heparin. To further characterize the molecular properties of the channel, we developed a novel assay to directly measure efflux of Ca2+ from intracellular stores of permeabilized Xenopus oocytes. Following size fractionation by sucrose gradient, poly(A)+ RNA from EA.hy926 cells is microinjected into oocytes of Xenopus laevis. We find that the mRNA encoding Ca2+ release activity is approximately 1.5-2.0 kilobases in length. The sphingolipid-gated Ca(2+)-permeable channel is thus likely to be a novel Ca(2+)-permeable channel distinct from other characterized intracellular Ca2+ channels such as the ryanodyne receptor and the inositol 1,4,5-trisphosphate receptor. The method described here provides a new approach to further characterizing this channel and other intracellular Ca2+ channels.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcimycin,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Heparin,
http://linkedlifedata.com/resource/pubmed/chemical/Nifedipine,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphorylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Sphingolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Sphingosine,
http://linkedlifedata.com/resource/pubmed/chemical/omega-Conotoxin GVIA,
http://linkedlifedata.com/resource/pubmed/chemical/sphingosine phosphorylcholine
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
270
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5266-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7890637-Animals,
pubmed-meshheading:7890637-Calcimycin,
pubmed-meshheading:7890637-Calcium,
pubmed-meshheading:7890637-Calcium Channel Blockers,
pubmed-meshheading:7890637-Calcium Channels,
pubmed-meshheading:7890637-Cell Line,
pubmed-meshheading:7890637-Endothelium, Vascular,
pubmed-meshheading:7890637-Female,
pubmed-meshheading:7890637-Heparin,
pubmed-meshheading:7890637-Humans,
pubmed-meshheading:7890637-Ion Channel Gating,
pubmed-meshheading:7890637-Kinetics,
pubmed-meshheading:7890637-Nifedipine,
pubmed-meshheading:7890637-Oocytes,
pubmed-meshheading:7890637-Peptides,
pubmed-meshheading:7890637-Phosphorylcholine,
pubmed-meshheading:7890637-RNA, Messenger,
pubmed-meshheading:7890637-Sphingolipids,
pubmed-meshheading:7890637-Sphingosine,
pubmed-meshheading:7890637-Time Factors,
pubmed-meshheading:7890637-Xenopus laevis,
pubmed-meshheading:7890637-omega-Conotoxin GVIA
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pubmed:year |
1995
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pubmed:articleTitle |
Sphingolipid-gated Ca2+ release from intracellular stores of endothelial cells is mediated by a novel Ca(2+)-permeable channel.
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pubmed:affiliation |
Cardiology Division, Duke University Medical Center, Durham, North Carolina 27710.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
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