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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1995-4-14
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pubmed:databankReference |
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pubmed:abstractText |
A monomeric rat beta-galactoside-binding lectin previously purified from extracts of rat lung has been localized to erythrocytes, and the cDNA encoding it has been isolated from a rat reticulocyte cDNA library. The deduced amino acid sequence of the cDNA predicts a protein with a M(r) of 16,199, with no evidence of a signal peptide. The deduced sequence is identical to the sequences of seven proteolytic peptides derived from the purified lectin. Peptide analysis by mass spectrometry indicates that the N-terminal methionine is cleaved and that serine 2 is acetylated. The lectin shares all the strictly conserved amino acid residues of other members of the mammalian galectin family and is designated galectin-5 (GenBank accession number L36862). Galectin-5 is a weak agglutinin of rat erythrocytes, despite its monomeric structure. The gene encoding galectin-5 (LGALS5) has been mapped in mouse to chromosome 11, approximately 50 centimorgans from the centromere and 1.8 +/- 1.8 centimorgans from the polymorphic marker D11Mit34n, a region syntenic with human chromosome 17q11.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
270
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5032-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7890611-Amino Acid Sequence,
pubmed-meshheading:7890611-Animals,
pubmed-meshheading:7890611-Base Sequence,
pubmed-meshheading:7890611-Chromatography, Gel,
pubmed-meshheading:7890611-Chromatography, High Pressure Liquid,
pubmed-meshheading:7890611-Cloning, Molecular,
pubmed-meshheading:7890611-DNA, Complementary,
pubmed-meshheading:7890611-DNA Primers,
pubmed-meshheading:7890611-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7890611-Erythrocytes,
pubmed-meshheading:7890611-Galectins,
pubmed-meshheading:7890611-Lectins,
pubmed-meshheading:7890611-Lung,
pubmed-meshheading:7890611-Mammals,
pubmed-meshheading:7890611-Molecular Sequence Data,
pubmed-meshheading:7890611-Open Reading Frames,
pubmed-meshheading:7890611-Peptide Fragments,
pubmed-meshheading:7890611-Polymerase Chain Reaction,
pubmed-meshheading:7890611-Protein Conformation,
pubmed-meshheading:7890611-Rats,
pubmed-meshheading:7890611-Recombinant Proteins,
pubmed-meshheading:7890611-Restriction Mapping,
pubmed-meshheading:7890611-Reticulocytes,
pubmed-meshheading:7890611-Sequence Homology, Amino Acid
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pubmed:year |
1995
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pubmed:articleTitle |
Sequence and mapping of galectin-5, a beta-galactoside-binding lectin, found in rat erythrocytes.
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pubmed:affiliation |
Department of Psychiatry, University of California, San Francisco 94143-0984.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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