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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
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pubmed:dateCreated |
1995-4-17
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pubmed:abstractText |
Two novel commercial IgG enzyme immunoassay (EIA) systems based on acid-glycine-extracted (Pyloriset IgG EIA, Orion Diagnostica) or fast protein liquid chromatography-purified (Cobas Core Anti-H. pylori EIA, Roche Diagnostic Systems) Helicobacter antigens were evaluated in a prospective study involving 127 patients. All patients underwent upper endoscopy with biopsy, and biopsies were examined for the presence of Helicobacter pylori by a rapid urease test, microscopy and culture. Of the 71 patients found to be infected with Helicobacter pylori, 69 (97.2%) and 65 (91.5%) tested positive with the Cobas Core and Pyloriset test, respectively. A detailed receiver operating characteristic analysis of the two tests showed that the Cobas Core assay was more sensitive and specific at every possible cut-off level; gave a better resolution of individual results, indicating a greater fine-sensitivity; and had no grey zone compared to a large grey zone encompassing 13.4% of the serum samples tested with the Pyloriset EIA. The Cobas Core assay appears to be a valuable tool for epidemiological purposes as well as for pre-endoscopic screening of dyspeptic patients.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0934-9723
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
13
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
812-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7889950-Antigens, Bacterial,
pubmed-meshheading:7889950-Blotting, Western,
pubmed-meshheading:7889950-Endoscopy, Gastrointestinal,
pubmed-meshheading:7889950-Helicobacter Infections,
pubmed-meshheading:7889950-Helicobacter pylori,
pubmed-meshheading:7889950-Humans,
pubmed-meshheading:7889950-Immunoenzyme Techniques,
pubmed-meshheading:7889950-Middle Aged,
pubmed-meshheading:7889950-Prospective Studies,
pubmed-meshheading:7889950-ROC Curve,
pubmed-meshheading:7889950-Sensitivity and Specificity
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pubmed:year |
1994
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pubmed:articleTitle |
Use of a receiver operating characteristic in the evaluation of two commercial enzyme immunoassays for detection of Helicobacter pylori infection.
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pubmed:affiliation |
Department of Bacteriology, University of Ulm, Germany.
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|