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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1995-4-20
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pubmed:abstractText |
A minus end-directed microtubule motor activity from extracts of HeLa cells blocked at prometaphase/metaphase of mitosis with vinblastine has been partially purified and characterized. The motor activity was eliminated by immunodepletion of Centromere binding protein E (CENP-E). The CENP-E-associated motor activity, which was not detectable in interphase cells, moved microtubules at mean rates of 0.46 micron/s at 37 degrees C and 0.24 micron/s at 25 degrees C. The motor activity co-purified with CENP-E through several purification procedures. Motor activity was clearly not due to dynein or to kinesin. The microtubule gliding rates of the CENP-E-associated motor were different from those of dynein and kinesin. In addition, the pattern of nucleotide substrate utilization by the CENP-E-associated motor and the sensitivity to inhibitors were different from those of dynein and kinesin. The CENP-E-associated motor had an apparent native molecular weight of 874,000 Da and estimated dimensions of 2 nm x 80 nm. This is the first demonstration of motor activity associated with CENP-E, strongly supporting the hypothesis that CENP-E may act as a minus end-directed microtubule motor during mitosis.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7889940-1253643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7889940-1406971,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/7889940-8506368
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
918-26
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7889940-Cell Movement,
pubmed-meshheading:7889940-Chromatography, DEAE-Cellulose,
pubmed-meshheading:7889940-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:7889940-Deoxyribonucleotides,
pubmed-meshheading:7889940-Dyneins,
pubmed-meshheading:7889940-HeLa Cells,
pubmed-meshheading:7889940-Humans,
pubmed-meshheading:7889940-Kinesin,
pubmed-meshheading:7889940-Metaphase,
pubmed-meshheading:7889940-Microtubules,
pubmed-meshheading:7889940-Mitosis,
pubmed-meshheading:7889940-Molecular Weight,
pubmed-meshheading:7889940-Ultraviolet Rays,
pubmed-meshheading:7889940-Vanadates
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pubmed:year |
1995
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pubmed:articleTitle |
Mitotic HeLa cells contain a CENP-E-associated minus end-directed microtubule motor.
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pubmed:affiliation |
Department of Biological Sciences, University of California, Santa Barbara 93106.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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