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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1995-4-18
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pubmed:abstractText |
Thymocyte differentiation proceeds from double positive CD4+CD8+ to single positive T cells. It has been proposed that this process occurs by an instructive or a stochastic mechanism. In this report, we show that in recombination-deficient mice (RAG-1-I-) constitutive expression of a CD8 transgene allows maturation of CD4+(CD8tg+) cells, which express mature levels of a transgenic class I-restricted T cell receptor, F5. Rescued F5+CD4+(CD8tg+) cells have equivalent levels of T cell receptor expression as CD8end+ cells, respond to cognate antigen and, upon stimulation, they exhibit a phenotype characteristic of CD4+ helper T cells. These data are consistent with a model of differentiation that predicts that thymocytes become functionally committed to a helper or cytotoxic lineage before the final step of positive selection and independently of MHC specificity of their T cell receptor.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1074-7613
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
1
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pubmed:geneSymbol |
RAG-1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
269-76
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7889414-Animals,
pubmed-meshheading:7889414-B-Lymphocytes,
pubmed-meshheading:7889414-Base Sequence,
pubmed-meshheading:7889414-CD4-Positive T-Lymphocytes,
pubmed-meshheading:7889414-CD8-Positive T-Lymphocytes,
pubmed-meshheading:7889414-Cell Differentiation,
pubmed-meshheading:7889414-Cytotoxicity, Immunologic,
pubmed-meshheading:7889414-DNA Primers,
pubmed-meshheading:7889414-Genes, MHC Class I,
pubmed-meshheading:7889414-Homeodomain Proteins,
pubmed-meshheading:7889414-Interleukin-4,
pubmed-meshheading:7889414-Lymphocyte Activation,
pubmed-meshheading:7889414-Mice,
pubmed-meshheading:7889414-Mice, Inbred C57BL,
pubmed-meshheading:7889414-Mice, Transgenic,
pubmed-meshheading:7889414-Molecular Sequence Data,
pubmed-meshheading:7889414-Peptides,
pubmed-meshheading:7889414-Proteins,
pubmed-meshheading:7889414-Receptors, Antigen, T-Cell,
pubmed-meshheading:7889414-T-Lymphocytes, Helper-Inducer
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pubmed:year |
1994
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pubmed:articleTitle |
Functional commitment to helper T cell lineage precedes positive selection and is independent of T cell receptor MHC specificity.
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pubmed:affiliation |
Laboratory of Molecular Immunology, National Institute for Medical Research, London, England.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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