| pubmed-article:7888165 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7888165 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:7888165 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:7888165 | lifeskim:mentions | umls-concept:C0013935 | lld:lifeskim |
| pubmed-article:7888165 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:7888165 | lifeskim:mentions | umls-concept:C1336633 | lld:lifeskim |
| pubmed-article:7888165 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:7888165 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:7888165 | pubmed:dateCreated | 1995-4-19 | lld:pubmed |
| pubmed-article:7888165 | pubmed:abstractText | Thymidine kinase (TK) activity was examined during the development of preimplantation mouse embryos. TK activity was increased approximately 20-fold from day 2 embryos (2-cell) to day 5 embryos (late blastocyst). TK activity did not change along with the progression into S-phase of the first and the second cell cycles but increased sharply at S-phase of the third cell cycle. Analysis of TK mRNA with a reverse transcription-polymerase chain reaction (RT-PCR) method showed that the level of TK mRNA was low in ovulated eggs and 1-cell embryos and was hardly detectable in day 2 embryos (2-cell), but sharply increased in day 3 embryos (mixture of 5- to 8-cell and morula). The functional role of 5'-flanking sequence of TK gene was also investigated in preimplantation embryos after microinjection with the DNA construct of 5'-flanking sequence of TK (2.4 kb) linked to bacterial lacZ gene (TK2.5lacZ) into the pronucleus of 1-cell and subsequently by histochemical staining with X-gal. beta-Galactosidase activity was first detected in day 3 embryos (8-cell), and 30% of embryos were stained with X-gal in day 4 and day 5 embryos, respectively. These results show that an increase in TK activity occurred after 2-cell stage, and this increase was primarily due to the embryonic activation of TK gene expression. Also, it appears that the 5'-flanking sequence of TK may directly regulate the TK gene expression at the transcriptional level during preimplantation murine development. | lld:pubmed |
| pubmed-article:7888165 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7888165 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7888165 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7888165 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7888165 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7888165 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7888165 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7888165 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:7888165 | pubmed:issn | 1040-452X | lld:pubmed |
| pubmed-article:7888165 | pubmed:author | pubmed-author:KimKK | lld:pubmed |
| pubmed-article:7888165 | pubmed:author | pubmed-author:ChoHH | lld:pubmed |
| pubmed-article:7888165 | pubmed:author | pubmed-author:LeeC CCC | lld:pubmed |
| pubmed-article:7888165 | pubmed:author | pubmed-author:LeeD KDK | lld:pubmed |
| pubmed-article:7888165 | pubmed:author | pubmed-author:RheeKK | lld:pubmed |
| pubmed-article:7888165 | pubmed:author | pubmed-author:SumPP | lld:pubmed |
| pubmed-article:7888165 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7888165 | pubmed:volume | 39 | lld:pubmed |
| pubmed-article:7888165 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7888165 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7888165 | pubmed:pagination | 259-67 | lld:pubmed |
| pubmed-article:7888165 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:7888165 | pubmed:meshHeading | pubmed-meshheading:7888165-... | lld:pubmed |
| pubmed-article:7888165 | pubmed:meshHeading | pubmed-meshheading:7888165-... | lld:pubmed |
| pubmed-article:7888165 | pubmed:meshHeading | pubmed-meshheading:7888165-... | lld:pubmed |
| pubmed-article:7888165 | pubmed:meshHeading | pubmed-meshheading:7888165-... | lld:pubmed |
| pubmed-article:7888165 | pubmed:meshHeading | pubmed-meshheading:7888165-... | lld:pubmed |
| pubmed-article:7888165 | pubmed:meshHeading | pubmed-meshheading:7888165-... | lld:pubmed |
| pubmed-article:7888165 | pubmed:meshHeading | pubmed-meshheading:7888165-... | lld:pubmed |
| pubmed-article:7888165 | pubmed:meshHeading | pubmed-meshheading:7888165-... | lld:pubmed |
| pubmed-article:7888165 | pubmed:meshHeading | pubmed-meshheading:7888165-... | lld:pubmed |
| pubmed-article:7888165 | pubmed:meshHeading | pubmed-meshheading:7888165-... | lld:pubmed |
| pubmed-article:7888165 | pubmed:meshHeading | pubmed-meshheading:7888165-... | lld:pubmed |
| pubmed-article:7888165 | pubmed:meshHeading | pubmed-meshheading:7888165-... | lld:pubmed |
| pubmed-article:7888165 | pubmed:meshHeading | pubmed-meshheading:7888165-... | lld:pubmed |
| pubmed-article:7888165 | pubmed:meshHeading | pubmed-meshheading:7888165-... | lld:pubmed |
| pubmed-article:7888165 | pubmed:meshHeading | pubmed-meshheading:7888165-... | lld:pubmed |
| pubmed-article:7888165 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7888165 | pubmed:articleTitle | Analysis of thymidine kinase gene expression in preimplantation mouse embryos. | lld:pubmed |
| pubmed-article:7888165 | pubmed:affiliation | Department of Molecular Biology, College of Natural Sciences, Seoul National University, Korea. | lld:pubmed |
| pubmed-article:7888165 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7888165 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:21877 | entrezgene:pubmed | pubmed-article:7888165 | lld:entrezgene |
