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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1995-4-10
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pubmed:abstractText |
pAM beta 1 is a low-copy-number, promiscuous plasmid from Gram-positive bacteria that replicates by a unidirectional theta-type mode. Its replication is initiated by an original mechanism, involving the positive rate-limiting RepE protein. Here we show that the pAM beta 1-encoded CopF protein is involved in negative regulation of the plasmid copy number. CopF represses approximately 10-fold the transcription initiated at the promoter of the repE gene and binds to a 31 bp segment which is located immediately upstream of the -35 box of the repE promoter. We propose that CopF inhibits initiation of transcription at the repE promoter by binding to its operator.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Nov
|
pubmed:issn |
0950-382X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:geneSymbol |
repE
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
463-71
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:7885230-Bacillus subtilis,
pubmed-meshheading:7885230-Base Sequence,
pubmed-meshheading:7885230-DNA, Bacterial,
pubmed-meshheading:7885230-Escherichia coli,
pubmed-meshheading:7885230-Gene Amplification,
pubmed-meshheading:7885230-Genes, Bacterial,
pubmed-meshheading:7885230-Genes, Regulator,
pubmed-meshheading:7885230-Molecular Sequence Data,
pubmed-meshheading:7885230-Operator Regions, Genetic,
pubmed-meshheading:7885230-Plasmids,
pubmed-meshheading:7885230-Promoter Regions, Genetic,
pubmed-meshheading:7885230-Transcription, Genetic
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pubmed:year |
1994
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pubmed:articleTitle |
The pAM beta 1 CopF repressor regulates plasmid copy number by controlling transcription of the repE gene.
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pubmed:affiliation |
Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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