Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
1995-4-7
|
pubmed:databankReference | |
pubmed:abstractText |
PCR was used to amplify approx. 1470-bp segments of DNA containing complete Shiga-like toxin type I (sltI) operons from Escherichia coli strains belonging to serotypes O48:H21, O111:H- and OX3:H8. These fragments were cloned and DNA sequence analysis identified several variations, as compared with published sltI sequences. All three sltI genes analysed were more closely related to Shiga toxin-encoding genes (sht) of Shigella dysenteriae type 1, than to previously published E. coli phage-encoded sltI genes. The greatest deviation in deduced amino acid (aa) sequence was observed in the SltI protein from the OX3:H8 strain, which differed from the phage 933J-encoded SltI by 9 aa in the A subunit and 3 aa in the B subunit.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Feb
|
pubmed:issn |
0378-1119
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
3
|
pubmed:volume |
153
|
pubmed:geneSymbol |
sltI
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
71-4
|
pubmed:dateRevised |
2008-11-21
|
pubmed:meshHeading |
pubmed-meshheading:7883188-Amino Acid Sequence,
pubmed-meshheading:7883188-Bacterial Toxins,
pubmed-meshheading:7883188-Base Sequence,
pubmed-meshheading:7883188-DNA, Bacterial,
pubmed-meshheading:7883188-Escherichia coli,
pubmed-meshheading:7883188-Genes, Bacterial,
pubmed-meshheading:7883188-Genetic Variation,
pubmed-meshheading:7883188-Molecular Sequence Data,
pubmed-meshheading:7883188-Operon,
pubmed-meshheading:7883188-Polymerase Chain Reaction,
pubmed-meshheading:7883188-Sequence Alignment,
pubmed-meshheading:7883188-Sequence Homology, Amino Acid,
pubmed-meshheading:7883188-Shiga Toxin 1
|
pubmed:year |
1995
|
pubmed:articleTitle |
Heterogeneity of the amino-acid sequences of Escherichia coli Shiga-like toxin type-I operons.
|
pubmed:affiliation |
Department of Microbiology, Women's and Children's Hospital, North Adelaide, Australia.
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|