Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1995-4-12
|
| pubmed:abstractText |
Insulin-dependent diabetes mellitus (IDDM) results from a T-cell-mediated destruction of the insulin-producing beta-cells. In this study, we designed a sensitive assay to detect and identify islet cell-reactive T-cells in patients with newly diagnosed IDDM. The relation between T-cell recognition of beta-cell antigens with IDDM and the pathogenesis of the disease (the beta-cell destruction process) was tested in a large group of IDDM patients and compared with T-cell responses in nondiabetic children with other chronic inflammations and in immunologically normal, age-matched control subjects. The results demonstrate that peripheral blood T-cells reacting with a beta-cell membrane preparation enriched for insulin-secretory granule antigen were detectable in the majority of newly diagnosed IDDM patients (27 of 40 [67%]; mean stimulation index [SI] 37.0). Such reactivity was reduced postonset in IDDM patients proportionally to the duration of the disease (11 of 30 [37%]; mean SI 8.7). Nondiabetic age-matched control subjects showed no responses or moderate responses to the granule preparation (4 of 48 [8%]; mean SI 3.4). The magnitude of the T-cell response was significantly greater in newly diagnosed IDDM patients than in IDDM patients tested at least 2 years postonset (P < 0.001). Two children in remission for insulin dependency (so-called honeymoon period) displayed exceptionally high proliferative responses to insulin-secretory granules (mean SI 86.7). These results imply that T-cell recognition of insulin-secretory granule antigens is associated with IDDM and in particular with the immune-mediated process of beta-cell destruction.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0012-1797
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
44
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
278-83
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7883114-Adolescent,
pubmed-meshheading:7883114-Adult,
pubmed-meshheading:7883114-Animals,
pubmed-meshheading:7883114-Antigens, Surface,
pubmed-meshheading:7883114-Cell Membrane,
pubmed-meshheading:7883114-Child,
pubmed-meshheading:7883114-Child, Preschool,
pubmed-meshheading:7883114-Cryopreservation,
pubmed-meshheading:7883114-Diabetes Mellitus, Type 1,
pubmed-meshheading:7883114-Female,
pubmed-meshheading:7883114-Humans,
pubmed-meshheading:7883114-Infant,
pubmed-meshheading:7883114-Inflammation,
pubmed-meshheading:7883114-Insulinoma,
pubmed-meshheading:7883114-Islets of Langerhans,
pubmed-meshheading:7883114-Lymphocyte Activation,
pubmed-meshheading:7883114-Male,
pubmed-meshheading:7883114-Pancreatic Neoplasms,
pubmed-meshheading:7883114-Rats,
pubmed-meshheading:7883114-Reference Values,
pubmed-meshheading:7883114-T-Lymphocytes,
pubmed-meshheading:7883114-Tetanus Toxoid
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
T-cell reactivity to beta-cell membrane antigens associated with beta-cell destruction in IDDM.
|
| pubmed:affiliation |
Department of Immunohaematology and Blood Bank, University Hospital, Leiden, The Netherlands.
|
| pubmed:publicationType |
Journal Article,
Clinical Trial,
Comparative Study,
Controlled Clinical Trial,
Research Support, Non-U.S. Gov't
|