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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1995-4-13
|
| pubmed:abstractText |
The mechanism of synergy between 3'-azido-3'-deoxythymidine (AZT) and anticancer agents was investigated with emphasis on cell-cycle events. Exposure of exponentially growing WiDr human colon carcinoma cells to AZT resulted in synchronization of cells in the S phase of the cell cycle. Following treatment with AZT at 50 or 200 microM, 62% +/- 3% or 82% +/- 4% of the cells were in the S phase as compared with 36% +/- 2% in the control. Bromodeoxyuridine uptake studies revealed that the synchronized cells actively synthesized DNA. At concentrations of up to 200 microM, AZT produced a cytostatic rather than cytotoxic effect as indicated by viability and cell growth measurements. At 200 microM, AZT-induced synchronization was significant (P = < 0.001) after 12 h of drug exposure, reached a maximum at 24 h, and reversed to baseline levels by 72 h even in the continued presence of the drug. This indicates that AZT-induced cytostasis is a transient and reversible effect. The cell-cycle events seen with AZT in WiDr cells were also observed in eight of nine human tumor cell lines tested. Isobologram analysis of WiDr cells preexposed to AZT for 24 h and then exposed to either AZT-5-fluorouracil or AZT-methotrexate for a further 72 h revealed synergy between AZT and the anticancer agents, indicating that AZT-induced synchronization may have therapeutic benefits.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorouracil,
http://linkedlifedata.com/resource/pubmed/chemical/Methotrexate,
http://linkedlifedata.com/resource/pubmed/chemical/Zidovudine
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0344-5704
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
35
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
489-95
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7882457-Antineoplastic Agents,
pubmed-meshheading:7882457-Breast Neoplasms,
pubmed-meshheading:7882457-Carcinoma,
pubmed-meshheading:7882457-Cell Division,
pubmed-meshheading:7882457-Cell Survival,
pubmed-meshheading:7882457-Colonic Neoplasms,
pubmed-meshheading:7882457-Computer Simulation,
pubmed-meshheading:7882457-DNA, Neoplasm,
pubmed-meshheading:7882457-Drug Synergism,
pubmed-meshheading:7882457-Flow Cytometry,
pubmed-meshheading:7882457-Fluorouracil,
pubmed-meshheading:7882457-Humans,
pubmed-meshheading:7882457-Leukemia,
pubmed-meshheading:7882457-Melanoma,
pubmed-meshheading:7882457-Methotrexate,
pubmed-meshheading:7882457-Pancreatic Neoplasms,
pubmed-meshheading:7882457-S Phase,
pubmed-meshheading:7882457-Tumor Cells, Cultured,
pubmed-meshheading:7882457-Zidovudine
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Synchronization of cells in the S phase of the cell cycle by 3'-azido-3'-deoxythymidine: implications for cell cytotoxicity.
|
| pubmed:affiliation |
Division of Cell Biology, Wellcome Research Laboratories, Research Triangle Park, NC 27709.
|
| pubmed:publicationType |
Journal Article,
Comparative Study
|