Linked Life Data

7882189

Source:http://linkedlifedata.com/resource/pubmed/id/7882189

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1995-4-12
pubmed:abstractText
To examine the effect of exercise on sarcoplasmic reticulum function in muscle tissue of different fibre composition, adult male Wistar rats weighing 388 +/- 23 g (x +/- SE) ran intermittently on a treadmill until fatigue. Fatigue was induced by 15-20 min of running performed at 52 m/min on an 8 degrees incline in periods of 2.5 min of exercise separated by 2 min of recovery. Analysis of sarcoplasmic reticulum Ca2+ ATPase activity determined in homogenates indicated no difference (p > 0.05) between age-matched control and exercised tissue for the soleus (SOL; 0.121 +/- 0.012 vs. 0.156 +/- 0.018 mumol.mg-1 protein.min-1), red gastrocnemius (RG; 0.381 +/- 0.022 vs. 0.354 +/- 0.022), or white gastrocnemius (WG; 0.526 +/- 0.05 vs. 0.471 +/- 0.031). Similarly, both total ATPase and Mg2+ ATPase activities were unaffected by the exercise in any of the tissues examined. Exercise also failed to alter sarcoplasmic reticulum Ca2+ uptake in homogenates of the SOL (1.43 +/- 0.15 vs. 1.38 +/- 0.19 nmol.mg-1 protein.min-1), RG (3.74 +/- 0.29 vs. 3.59 +/- 0.24), and WG (5.98 +/- 0.48 vs. 5.41 +/- 0.50). At fatigue, glycogen depletion was similar in all tissue types and amounted to 65.1% in the SOL (172 +/- 9 vs. 60 +/- 16 mmol.glucosyl units-1.kg-1 dry weight), 74.4% in RG (164 +/- 8 vs. 42 +/- 6), and 79% in the WG (167 +/- 9 vs. 35 +/- 9). It is concluded that exercise by itself does not alter sarcoplasmic reticulum Ca2(+)-sequestering function in tissues of primarily different fibre composition when determined in homogenates in vitro.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0008-4212
pubmed:author
pubmed:issnType
Print
pubmed:volume
72
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1231-7
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Preservation of sarcoplasmic reticulum Ca2(+)-sequestering function in homogenates of different type composition following sprint activity.
pubmed:affiliation
Department of Kinesiology, University of Waterloo, ON, Canada.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, Non-U.S. Gov't