Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1995-4-12
pubmed:abstractText
The unique availability of an explanted liver-islet allograft, removed for primary nonfunction of the liver, led us to evaluate distribution and phenotype of exocrine and endocrine components of the pancreatic graft. Immunocytochemistry was used to map patterns of gene products for islet hormones, proprotein processing enzymes, panneuroendocrine markers, and pancreatic exocrine markers. When compared with age-matched control pancreases, insulin-, glucagon-, somatostatin-, and pancreatic polypeptide-producing cells were similarly represented and distributed within the grafted islet. We also demonstrate that the intrahepatic transplanted islets retained the enzyme machinery able to process the hormone precursors into bioactive fragments. In the clinical setting, this resulted in an immediate functioning of the graft and insulin-independence of the patient one month after transplantation. The purity in islets, as assessed by immunocytochemistry with antibodies to tissue constituents of endocrine and exocrine lineages, was around 40%. Despite the massive intraportal presence of pancreatic acinar tissue, no signs or symptoms attributable to ectopic hypersecretion of exocrine enzymes occurred. In fact, when tested with antibodies to such enzymes, low levels of immunoreactivity were observed in the grafted acinar cells.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers, http://linkedlifedata.com/resource/pubmed/chemical/Carboxypeptidase H, http://linkedlifedata.com/resource/pubmed/chemical/Carboxypeptidases, http://linkedlifedata.com/resource/pubmed/chemical/Glucagon, http://linkedlifedata.com/resource/pubmed/chemical/Insulin, http://linkedlifedata.com/resource/pubmed/chemical/Mixed Function Oxygenases, http://linkedlifedata.com/resource/pubmed/chemical/Multienzyme Complexes, http://linkedlifedata.com/resource/pubmed/chemical/Pancreatic Polypeptide, http://linkedlifedata.com/resource/pubmed/chemical/Proinsulin, http://linkedlifedata.com/resource/pubmed/chemical/Somatostatin, http://linkedlifedata.com/resource/pubmed/chemical/Subtilisins, http://linkedlifedata.com/resource/pubmed/chemical/peptidylglycine monooxygenase
pubmed:status
MEDLINE
pubmed:issn
0963-6897
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
N
pubmed:pagination
499-508
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:7881762-Biological Markers, pubmed-meshheading:7881762-Carboxypeptidase H, pubmed-meshheading:7881762-Carboxypeptidases, pubmed-meshheading:7881762-Carcinoma, Neuroendocrine, pubmed-meshheading:7881762-Glucagon, pubmed-meshheading:7881762-Humans, pubmed-meshheading:7881762-Immunohistochemistry, pubmed-meshheading:7881762-Insulin, pubmed-meshheading:7881762-Islets of Langerhans Transplantation, pubmed-meshheading:7881762-Liver Neoplasms, pubmed-meshheading:7881762-Liver Transplantation, pubmed-meshheading:7881762-Male, pubmed-meshheading:7881762-Middle Aged, pubmed-meshheading:7881762-Mixed Function Oxygenases, pubmed-meshheading:7881762-Multienzyme Complexes, pubmed-meshheading:7881762-Pancreatic Neoplasms, pubmed-meshheading:7881762-Pancreatic Polypeptide, pubmed-meshheading:7881762-Proinsulin, pubmed-meshheading:7881762-Reoperation, pubmed-meshheading:7881762-Somatostatin, pubmed-meshheading:7881762-Subtilisins, pubmed-meshheading:7881762-Transplantation, Heterotopic, pubmed-meshheading:7881762-Transplantation, Homologous
pubmed:articleTitle
Immunocytochemical detection and characterization of intrahepatic human pancreatic islets after combined liver-islet allotransplantation.
pubmed:affiliation
Department of Pathology, Istituto Nazionale Tumori, Milan, Italy.
pubmed:publicationType
Journal Article, Comparative Study, Case Reports, Research Support, Non-U.S. Gov't