Linked Life Data

7881128

Source:http://linkedlifedata.com/resource/pubmed/id/7881128

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-4-7
pubmed:abstractText
Molecules regulating morphogenesis by cell-cell interactions are the cadherins, a class of calcium-dependent adhesion molecules. One of its members, M-cadherin, has been isolated from a myoblast cell line (Donalies et al. [1991] Proc. Natl. Acad. Sci. U.S.A. 88:8024-8028). In mouse development, expression of M-cadherin mRNA first appears at day 8.5 of gestation (E8.5) in somites and has been postulated to be down-regulated in developing muscle masses (Moore and Walsh [1993] Development 117:1409-1420). Affinity-purified polyclonal M-cadherin antibodies, detecting a protein of approximately 120 kDa, were used to study the cell expression pattern of M-cadherin protein. It was first visualized in somites at E10 1/3 and could be confined to desmin positive, myotomal cells. At all subsequent prenatal stages, M-cadherin was only found in myogenic cells of somitic origin. The detection of the protein at E10 1/3 suggests a translational delay of M-cadherin mRNA of 1 to 2 days (E8.5 vs. E10 1/3). This was further supported by the finding that during differentiation of ES cell line BLC6 into skeletal muscle cells in culture, expression of M-cadherin mRNA can be detected 2 days prior to M-cadherin protein. During prenatal development, the pattern of M-cadherin expression changes: In E10 1/3 embryos and also in myotomal cells of later stages, M-cadherin is evenly distributed on the cell surface. In developing muscle masses (tested at E16 to E18), however, M-cadherin protein becomes clustered most likely at sites of cell-cell contact as indicated by double-labelling experiments: M-cadherin-staining is the positive image of laminin negative areas excluding the presence of a basal lamina at M-cadherin positive sites. Furthermore, M-cadherin is coexpressed with the neuronal cell adhesion molecule N-CAM which has been shown to mediate cell-cell contact in myogenic cells. In summary, our results are in line with the idea that M-cadherin might play a central role in myogenic morphogenesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1058-8388
pubmed:author
pubmed:issnType
Print
pubmed:volume
201
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
245-59
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7881128-Amino Acid Sequence, pubmed-meshheading:7881128-Animals, pubmed-meshheading:7881128-Base Sequence, pubmed-meshheading:7881128-Cadherins, pubmed-meshheading:7881128-Cell Adhesion Molecules, Neuronal, pubmed-meshheading:7881128-Cell Membrane, pubmed-meshheading:7881128-Cells, Cultured, pubmed-meshheading:7881128-DNA Primers, pubmed-meshheading:7881128-Embryonic and Fetal Development, pubmed-meshheading:7881128-Female, pubmed-meshheading:7881128-Fluorescent Antibody Technique, pubmed-meshheading:7881128-Gene Expression Regulation, Developmental, pubmed-meshheading:7881128-Gestational Age, pubmed-meshheading:7881128-Mice, pubmed-meshheading:7881128-Mice, Inbred BALB C, pubmed-meshheading:7881128-Molecular Sequence Data, pubmed-meshheading:7881128-Muscle, Skeletal, pubmed-meshheading:7881128-Pregnancy, pubmed-meshheading:7881128-RNA, Messenger, pubmed-meshheading:7881128-Recombinant Fusion Proteins, pubmed-meshheading:7881128-Stem Cells
pubmed:year
1994
pubmed:articleTitle
Expression of M-cadherin protein in myogenic cells during prenatal mouse development and differentiation of embryonic stem cells in culture.
pubmed:affiliation
Institut der Anthropologie und Humangenetik für Biologen, Johann Wolfgang Goethe-Universität, Frankfurt am Main, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't