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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1995-4-4
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pubmed:abstractText |
The immunogold labeling technique and transmission electron microscopy were used to demonstrate the expression and position of the intermediate filament vimentin in rat osteoblast and osteocyte cell bodies and cell processes. Conventional light and transmission electron microscopic studies of bone cells demonstrated adjacent cell linkage to be mediated by osteoblast and osteocyte processes present within the canalicular system traversing the bone matrix. The cell processes were filled with densely packed filaments, many of which have been shown previously to be actin microfilaments. The appearance, however, of 10 nm diameter filaments in some cell processes and the fact the intermediate filament vimentin has been defined in many cells of mesenchymal origin raised the possibility that some of these filaments might be vimentin. The ultrastructural colloidal gold immunochemical technique allowed for demonstration in situ of the expression of vimentin filaments plus accurate definition of their position.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0003-276X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
241
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
39-48
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7879923-Age Factors,
pubmed-meshheading:7879923-Animals,
pubmed-meshheading:7879923-Animals, Newborn,
pubmed-meshheading:7879923-Bone Matrix,
pubmed-meshheading:7879923-Gap Junctions,
pubmed-meshheading:7879923-Immunohistochemistry,
pubmed-meshheading:7879923-Microscopy, Electron,
pubmed-meshheading:7879923-Osteoblasts,
pubmed-meshheading:7879923-Osteocytes,
pubmed-meshheading:7879923-Rats,
pubmed-meshheading:7879923-Rats, Sprague-Dawley,
pubmed-meshheading:7879923-Vimentin
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pubmed:year |
1995
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pubmed:articleTitle |
Transmission electron microscopic demonstration of vimentin in rat osteoblast and osteocyte cell bodies and processes using the immunogold technique.
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pubmed:affiliation |
Department of Orthopaedic Surgery, Harvard Medical School, Children's Hospital, Boston, Massachusetts 02115.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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