Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1995-4-4
pubmed:abstractText
The molecular mechanisms responsible for hepatocyte death and the events leading to viral clearance in hepatitis B virus (HBV) infections are not well understood. Elucidation of the mechanisms involved have been complicated by the difficulty of infecting human hepatocytes with HBV in vitro and the lack of an appropriate animal model. We report an animal model of human HBV infection by in vivo transfection. We have directly introduced a replication-competent, cloned HBV construct into rat liver by using a membrane fusion-promoting cationic lipid. HBV mRNA and 3.2-kb HBV DNA were expressed in the liver by this in vivo transfection method. In the majority of rats, HBV virions and hepatitis B e antigen were found in the blood 3-7 days after transfection, after which antibody to the e antigen appeared. Two to three weeks after the transfection, glutamic-pyruvic transaminase levels were elevated in serum, hepatocyte death and lymphocyte infiltration were observed in the vicinity of the portal vein of liver, and HBV virions were no longer detected in the serum. Thus, transfection of HBV into rats resulted in histological and serological changes comparable to HBV-induced acute hepatitis in humans. In contrast, no hepatocellular injury was observed in T-lymphocyte-deficient nude rats transfected with the same HBV construct, and viremia was substantially prolonged, providing direct evidence that T lymphocytes play an essential role in liver cell injury and in the clearance of HBV. This rat hepatitis model will be useful for studying pathogenesis of HBV infection.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-1316145, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-1583471, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-1691527, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-2002544, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-2034246, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-2034247, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-2066074, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-2538818, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-2570285, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-2671579, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-2762328, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-2780554, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-2793840, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-2794069, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-2822953, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-2843594, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-2995835, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-3019565, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-3029758, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-3033659, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-3172341, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-3282484, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-3290682, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-6116901, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-6618432, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-6982941, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-7679709, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-7687073, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-8228807, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-8399362, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-8441471, http://linkedlifedata.com/resource/pubmed/commentcorrection/7878002-8496181
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
92
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1470-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:7878002-Acute Disease, pubmed-meshheading:7878002-Alanine Transaminase, pubmed-meshheading:7878002-Animals, pubmed-meshheading:7878002-Animals, Genetically Modified, pubmed-meshheading:7878002-Base Sequence, pubmed-meshheading:7878002-DNA Primers, pubmed-meshheading:7878002-Disease Models, Animal, pubmed-meshheading:7878002-Hepatitis, Viral, Animal, pubmed-meshheading:7878002-Hepatitis B Antibodies, pubmed-meshheading:7878002-Hepatitis B e Antigens, pubmed-meshheading:7878002-Hepatitis B virus, pubmed-meshheading:7878002-Liver, pubmed-meshheading:7878002-Molecular Sequence Data, pubmed-meshheading:7878002-RNA, Viral, pubmed-meshheading:7878002-Rats, pubmed-meshheading:7878002-Rats, Nude, pubmed-meshheading:7878002-Rats, Sprague-Dawley, pubmed-meshheading:7878002-Transfection
pubmed:year
1995
pubmed:articleTitle
Acute hepatitis in rats expressing human hepatitis B virus transgenes.
pubmed:affiliation
Gastrointestinal Unit, Harvard Medical School, Boston, MA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.