Linked Life Data

7876030

Source:http://linkedlifedata.com/resource/pubmed/id/7876030

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-4-6
pubmed:abstractText
Fluorescence in situ hybridization with whole chromosome libraries, also known as chromosome "painting", is an easy and rapid method for detection of chromosome aberrations. To evaluate the sensitivity of this in radiation dosimetry we have made comparisons with G-banding analysis and also with physicochemical measurements of radiation induced DNA damage (DNA strand breaks and 8-hydroxydeoxyguanosine formation). Heparinised human blood was irradiated at room temperature with a range (0-10 Gy) of gamma irradiation from a cobalt 60 source. Chromosome spreads prepared from phytohaemagglutinin stimulated "whole blood" lymphocyte cultures were hybridized in situ with the whole chromosome 1 library, coded, and scored for aberrant cells. Dose response curves plotted as percent abnormal cells obtained by the two cytogenetic methods were similar and it would appear that chromosome "painting" compared favourably with G-banding for the detection of aberrations. The measurement of DNA strand breaks by a fluorimetric alkaline unwinding assay showed similar sensitivity to chromosome "painting" whereas the formation of 8-hydroxydeoxyguanosine did not correlate with aberration frequencies.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0018-0661
pubmed:author
pubmed:issnType
Print
pubmed:volume
121
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
139-45
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Evaluation of the relative sensitivity of chromosome painting (FISH) as an indicator of radiation-induced damage in human lymphocytes.
pubmed:affiliation
LFS Research Unit, Birmingham Heartlands Hospital, U.K.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't