Linked Life Data

7869586

Source:http://linkedlifedata.com/resource/pubmed/id/7869586

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1995-3-28
pubmed:databankReference
pubmed:abstractText
We used a method for molecular typing of the methicillin resistance determinant (mec) based on the size of the mec-associated hypervariable region amplified by the polymerase chain reaction (PCR) to examine 106 methicillin-resistant Staphylococcus aureus (MRSA), 9 methicillin-resistant (Mcr) S. epidermidis and 5 Mcr S. haemolyticus clinical isolates. In the 106 MRSA isolates, 5 sizes of PCR products were observed. The MRSA isolates were grouped into five hypervariable region (HVR) genotypes on the basis of the size of the PCR product. The PCR products amplified from 8 of 9 Mcr S. epidermidis isolates were the same as products amplified from MRSA isolates, which was confirmed by the PCR-SSCP (single-strand conformation polymorphism) method. In 32 methicillin-susceptible isolates, the target gene was not amplified. This method is thought to be useful in epidemiological investigations of nosocomial infections caused by MRSA. This is the first typing method capable of comparing the mec determinants of MRSA isolates and Mcr CNS isolates to establish the origin of the mec determinant.
pubmed:language
jpn
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0047-1860
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:geneSymbol
mecA
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1227-33
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
[Molecular typing of the methicillin resistance determinant (mec) of clinical strains of Staphylococcus based on mec hypervariable region length polymorphisms].
pubmed:affiliation
Department of Pediatrics, Kagoshima University.
pubmed:publicationType
Journal Article, English Abstract