Linked Life Data

786893

Source:http://linkedlifedata.com/resource/pubmed/id/786893

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1976-12-1
pubmed:abstractText
Streptococcal pyrogenic exotoxin (SPE) isolated from culture filtrates of strain NY-5 (type 10), and separated from other extracellular by differential solubility in ethanol and acetate-buffered saline, has previously been shown to exhibit a wide range of biological activities including erythrogenic activity, pyrogenicity, enhancement of susceptibility to endotoxin shock, blockage of the reticuloendothelial system immmunosuppression, and lymphocyte mitogenicity. Toxin prepared in this way was found to consist of hyaluronic acid and several proteins which could be distinguished by thin-layer polyacrylamide isoelectric focusing (IEF), SPE has been further purified by ion exchange chromatography on QAE-Sephadex columns. One of the fractions isolated from QAE-Sephadex, and shown to be a homogenous protein by thin-layer IEF and Ouchterlony with hyperimmune serum, was highly active erythrogenically, pyrogenically, and in enhancing susceptibility to endotoxin. This fraction was identified as exotoxin A. A second, less active fraction identified as SPE B showed similar activities, but differed from the other fraction antigenically and in net charge and molecular weight. These findings indicate that a single highly purified protein can mediate at least three of the biological activities attributed to SPE and NY-5 produces pyrogenic exotoxins A and B in vitro as well as in vivo.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/786893-1089599, http://linkedlifedata.com/resource/pubmed/commentcorrection/786893-13539304, http://linkedlifedata.com/resource/pubmed/commentcorrection/786893-13783427, http://linkedlifedata.com/resource/pubmed/commentcorrection/786893-13931033, http://linkedlifedata.com/resource/pubmed/commentcorrection/786893-14155091, http://linkedlifedata.com/resource/pubmed/commentcorrection/786893-14255656, http://linkedlifedata.com/resource/pubmed/commentcorrection/786893-4187595, http://linkedlifedata.com/resource/pubmed/commentcorrection/786893-4227774, http://linkedlifedata.com/resource/pubmed/commentcorrection/786893-4577414, http://linkedlifedata.com/resource/pubmed/commentcorrection/786893-4578079, http://linkedlifedata.com/resource/pubmed/commentcorrection/786893-4866097, http://linkedlifedata.com/resource/pubmed/commentcorrection/786893-4905084, http://linkedlifedata.com/resource/pubmed/commentcorrection/786893-4982089
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
767-75
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
Further purification of group A streptococcal pyrogenic exotoxin and characterization of the purified toxin.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.