Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1976-12-3
pubmed:abstractText
A partially purified enterotoxin was obtained from the growth medium of Escherichia coli strain 711 (P307), a derivative of E. coli K-12, by ultrafiltration, precipitation with ammonium sulfate, molecular sieving, and anion exchange column chromatography. The active moiety, which is heat-labile, behaved like a protein particle of 180,000 to 200,000 daltons during molecular sieving and ultracentrifugation. During polyacrylamide gel electrophoresis in sodium dodecyl sulfate (SDS-PAGE), it dissociated into two subunits with apparent molecular weights of 68,000 to 70,000 and 14,000 to 15,000. SDS-PAGE after heating in SDS changed the larger subunit to an apparent molecular weight of about 40,000; the smaller subunit did not change. The intact particle induced rounding of the cells in Y-1 mouse adrenal tumor cells used for assay. The detergent-dissociated molecules were not active. Proteolysis of the purified toxin by tolylsulfonyl phenylalanyl chloromethyl ketone-trypsin appeared to enhance its activity. The addition of serum to the assay medium resulted in partial depression of the activity. Activity was also abolished by preincubation of the toxin with either a rabbit antiserum to it or solutions containing GM1 ganglioside. The length of time needed to evoke a response in the assay system by fractions from different stages in the purification of the enterotoxin was a useful parameter in the evaluation of specific activity.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/786881-1104580, http://linkedlifedata.com/resource/pubmed/commentcorrection/786881-1193729, http://linkedlifedata.com/resource/pubmed/commentcorrection/786881-13945475, http://linkedlifedata.com/resource/pubmed/commentcorrection/786881-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/786881-241754, http://linkedlifedata.com/resource/pubmed/commentcorrection/786881-4155864, http://linkedlifedata.com/resource/pubmed/commentcorrection/786881-4199382, http://linkedlifedata.com/resource/pubmed/commentcorrection/786881-4199562, http://linkedlifedata.com/resource/pubmed/commentcorrection/786881-4206342, http://linkedlifedata.com/resource/pubmed/commentcorrection/786881-4366919, http://linkedlifedata.com/resource/pubmed/commentcorrection/786881-4584048, http://linkedlifedata.com/resource/pubmed/commentcorrection/786881-4587905, http://linkedlifedata.com/resource/pubmed/commentcorrection/786881-4593468, http://linkedlifedata.com/resource/pubmed/commentcorrection/786881-4601180, http://linkedlifedata.com/resource/pubmed/commentcorrection/786881-4633289, http://linkedlifedata.com/resource/pubmed/commentcorrection/786881-4861401, http://linkedlifedata.com/resource/pubmed/commentcorrection/786881-4919579, http://linkedlifedata.com/resource/pubmed/commentcorrection/786881-4919580, http://linkedlifedata.com/resource/pubmed/commentcorrection/786881-4979520, http://linkedlifedata.com/resource/pubmed/commentcorrection/786881-5143846, http://linkedlifedata.com/resource/pubmed/commentcorrection/786881-5806584
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1710-20
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
Partial purification and characterization of a heat-labile enterotoxin of Escherichia coli.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.