Linked Life Data

7866511

Source:http://linkedlifedata.com/resource/pubmed/id/7866511

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-3-29
pubmed:abstractText
Ribose-methylated dinucleotides of the type NmpN' derived from digestion of tRNA with RNase T2 were separated and characterized by directly combined liquid chromatography--mass spectrometry (LC-MS) with a continuous-flow frit-fast atom bombardment (frit-FAB) interface. Prediction of NmpN' peaks was readily made by comparison of the LC profile with that of comparative nuclease P1 digest. The identity of the candidate peaks including NmpN' was further recognized by the mass spectra, in which NmpN' showed intense molecular-related ions, in addition to sequence-specific fragment ions, to verify the chemical structures in both positive- and negative-ion modes. The method was applied to screening NmpN' (and NmpN' mpN") in tRNA from the extremely thermophilic archaeon Pyrodictium occultum.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1572-6495
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
660
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
223-33
pubmed:dateRevised
2007-10-16
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Detection of ribose-methylated nucleotides in Pyrodictium occultum tRNA by liquid chromatography--frit-fast atom bombardment mass spectrometry.
pubmed:affiliation
Faculty of Pharmacy, Meijo University, Nagoya, Japan.
pubmed:publicationType
Journal Article