Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-3-30
pubmed:abstractText
Human bronchial surface epithelial cells were maintained in secondary culture on a collagen gel substrate in a defined, serum-free medium. These conditions have previously been reported to promote mucous cell differentiation. After 3 wk in culture, approximately 40% of the cells were stained by an antibody directed against human respiratory mucin. Analysis of media from cells cultured in the presence of the radioactive precursors [3H]glucosamine and [35S]sulfate revealed that the cells secreted high molecular weight glycoproteins with properties of typical respiratory mucins. In addition, hyaluronic acid and proteoglycans containing chondroitin sulfate and/or heparan sulfate glycosaminoglycans were identified in cell conditioned media. Finally, Western blot analyses showed that the cells secreted lysozyme and mucous proteinase inhibitor, proteins that are generally considered to be markers for submucosal gland serous cells. These results show that human bronchial cells from the surface epithelium in secondary culture secreted a range of glycoconjugates and proteins that were typical secretory products of both mucous and serous cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1044-1549
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
130-41
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7865212-Bronchi, pubmed-meshheading:7865212-Cell Differentiation, pubmed-meshheading:7865212-Cells, Cultured, pubmed-meshheading:7865212-Chondroitin Sulfates, pubmed-meshheading:7865212-Culture Media, pubmed-meshheading:7865212-Cytological Techniques, pubmed-meshheading:7865212-Epithelium, pubmed-meshheading:7865212-Glycoconjugates, pubmed-meshheading:7865212-Heparitin Sulfate, pubmed-meshheading:7865212-Humans, pubmed-meshheading:7865212-Hyaluronic Acid, pubmed-meshheading:7865212-Microscopy, Electron, pubmed-meshheading:7865212-Mucins, pubmed-meshheading:7865212-Mucus, pubmed-meshheading:7865212-Muramidase, pubmed-meshheading:7865212-Proteinase Inhibitory Proteins, Secretory, pubmed-meshheading:7865212-Proteins, pubmed-meshheading:7865212-Proteoglycans, pubmed-meshheading:7865212-Serine Proteinase Inhibitors
pubmed:year
1995
pubmed:articleTitle
Mucous and serous secretions of human bronchial epithelial cells in secondary culture.
pubmed:affiliation
Unité INSERM No. 377, Lille, France.
pubmed:publicationType
Journal Article