Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2 Pt 1
pubmed:dateCreated
1995-3-21
pubmed:abstractText
The effect and possible signal transduction pathway of interleukin-1 beta (IL-1 beta) on the L-type Ca2+ current (ICa,L) in cultured adult rat ventricular myocytes were examined using whole cell patch-clamp techniques. When myocytes were internally dialyzed with a solution containing GTP, IL-1 beta caused a concentration-dependent decrease in the peak ICa,L (Ba2+ as the charge carrier). IL-1 beta did not significantly alter the voltage dependence of the peak ICa,L nor the steady-state inactivation and activation, but did slightly slow the rate of inactivation. In myocytes dialyzed with solutions without GTP or including guanosine 5'-O-(2-thiodiphosphate) to replace GTP, IL-1 beta had no effect on ICa,L. In contrast, when guanosine 5'-O-(3-thiotriphosphate) was used to replace GTP, the suppression of ICa,L induced by IL-1 beta remained. Preincubation of myocytes with pertussis toxin (PTX), which completely abolished the acetylcholine effect on isoproterenol-stimulated ICa,L, had no effect on the inhibitory action of IL-1 beta on ICa,L. We conclude that in cultured rat ventricular myocytes, IL-1 beta suppresses ICa,L via a PTX-insensitive G protein.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
268
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C339-49
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7864073-Animals, pubmed-meshheading:7864073-Barium, pubmed-meshheading:7864073-Calcium, pubmed-meshheading:7864073-Calcium Channel Blockers, pubmed-meshheading:7864073-Cells, Cultured, pubmed-meshheading:7864073-Electric Conductivity, pubmed-meshheading:7864073-GTP-Binding Proteins, pubmed-meshheading:7864073-Guanine Nucleotides, pubmed-meshheading:7864073-Heart, pubmed-meshheading:7864073-Heart Ventricles, pubmed-meshheading:7864073-Homeostasis, pubmed-meshheading:7864073-Interleukin-1, pubmed-meshheading:7864073-Kinetics, pubmed-meshheading:7864073-Male, pubmed-meshheading:7864073-Myocardium, pubmed-meshheading:7864073-Pertussis Toxin, pubmed-meshheading:7864073-Rats, pubmed-meshheading:7864073-Rats, Sprague-Dawley, pubmed-meshheading:7864073-Virulence Factors, Bordetella
pubmed:year
1995
pubmed:articleTitle
G protein-mediated suppression of L-type Ca2+ current by interleukin-1 beta in cultured rat ventricular myocytes.
pubmed:affiliation
Department of Medicine, University of Arkansas for Medical Sciences, Little Rock 72205.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't