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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1995-3-20
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pubmed:abstractText |
We have developed a transgenic approach, termed immunotoxin-mediated cell targeting (IMCT), to ablate conditionally selective neurons in the brain with the cytotoxic activity of immunotoxins. Transgenic mice were created that express the human interleukin 2 receptor alpha subunit (IL-2R alpha) under the control of the dopamine beta-hydroxylase (DBH) gene promoter. The animals were treated intracerebroventricularly with a recombinant immunotoxin, anti-Tac(Fv)-PE40, which selectively kills animal cells bearing human IL-2R alpha. The immunotoxin caused a characteristic behavioral abnormality only in the transgenic mice. This was accompanied by a dramatic loss of DBH-containing neurons and a significant decrease in DBH activity and norepinephrine levels in various regions of the brain. IMCT should provide a general technique to create animal models of human neurodegenerative disorders by targeting neurons or other cell types.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-1497314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-1542654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-1696727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-1970511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-2118522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-2304463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-2498664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-2539597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-2656404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-2733785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-2845412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-298352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-3078520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-3529238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-3536124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-3649277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-3685993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-4376244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-4395919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-5544323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-6090949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-6823561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-6970774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-7027315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-7961964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-8013566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862648-8510498
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ADP Ribose Transferases,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Toxins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Dopamine beta-Hydroxylase,
http://linkedlifedata.com/resource/pubmed/chemical/Exotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors,
http://linkedlifedata.com/resource/pubmed/chemical/toxA protein, Pseudomonas aeruginosa
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
92
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1132-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7862648-ADP Ribose Transferases,
pubmed-meshheading:7862648-Animals,
pubmed-meshheading:7862648-Bacterial Toxins,
pubmed-meshheading:7862648-Base Sequence,
pubmed-meshheading:7862648-DNA Primers,
pubmed-meshheading:7862648-Dopamine beta-Hydroxylase,
pubmed-meshheading:7862648-Exotoxins,
pubmed-meshheading:7862648-Humans,
pubmed-meshheading:7862648-Immunotoxins,
pubmed-meshheading:7862648-Mice,
pubmed-meshheading:7862648-Mice, Transgenic,
pubmed-meshheading:7862648-Molecular Sequence Data,
pubmed-meshheading:7862648-Neurons,
pubmed-meshheading:7862648-Promoter Regions, Genetic,
pubmed-meshheading:7862648-Receptors, Interleukin-2,
pubmed-meshheading:7862648-Virulence Factors
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pubmed:year |
1995
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pubmed:articleTitle |
Immunotoxin-mediated conditional disruption of specific neurons in transgenic mice.
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pubmed:affiliation |
Institute for Comprehensive Medical Science, School of Medicine, Fujita Health University, Toyoake, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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