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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1995-3-23
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pubmed:abstractText |
Candida albicans WO-1 switches spontaneously, frequently, and reversibly between a hemispherical white and a flat gray (opaque) colony-forming phenotype. This transition affects a number of morphological and physiological parameters and involves the activation and deactivation of phase-specific genes. The WH11 gene is transcribed in the white but not the opaque phase. A chimeric WH11-firefly luciferase gene containing the 5' upstream region of WH11 was demonstrated to be under phase regulation regardless of the site of integration, and a series of promoter deletion constructs was used to delineate two white-phase-specific transcription activation domains. Gel retardation experiments with the individual distal or proximal domain and white-phase or opaque-phase protein extract demonstrated the formation of one distal white-phase-specific complex and two proximal white-phase-specific complexes. Specific subfragments were tested for their ability to compete with the entire domain in the formation of complexes with white-phase protein extract in order to map the proximal domain sequence involved in white-phase-specific complex formation. Our results indicate that white-phase-specific transcription of WH11 is positively regulated by trans-acting factors interacting with two cis-acting activation sequences in the WH11 promoter.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862169-1448073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862169-1576587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862169-1620110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862169-1880680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862169-2129531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862169-2196431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862169-2403540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862169-2463158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862169-2725487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862169-3053160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862169-3062587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862169-3201230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862169-3280141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862169-3290688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862169-3315231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862169-3316187,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/7862169-387610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862169-3901258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862169-3903043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862169-6326095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7862169-7016556,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/7862169-8478072
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1797-805
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7862169-Animals,
pubmed-meshheading:7862169-Base Sequence,
pubmed-meshheading:7862169-Beetles,
pubmed-meshheading:7862169-Candida albicans,
pubmed-meshheading:7862169-Fungal Proteins,
pubmed-meshheading:7862169-Gene Expression Regulation, Fungal,
pubmed-meshheading:7862169-Genes, Fungal,
pubmed-meshheading:7862169-Luciferases,
pubmed-meshheading:7862169-Molecular Sequence Data,
pubmed-meshheading:7862169-Promoter Regions, Genetic,
pubmed-meshheading:7862169-Recombinant Fusion Proteins,
pubmed-meshheading:7862169-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:7862169-Sequence Deletion,
pubmed-meshheading:7862169-Transcription, Genetic
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pubmed:year |
1995
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pubmed:articleTitle |
Functional analysis of the promoter of the phase-specific WH11 gene of Candida albicans.
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pubmed:affiliation |
Department of Biological Sciences, University of Iowa, Iowa City 52242.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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