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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1995-3-17
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pubmed:abstractText |
The large intracellular loop (IL) of the gamma 2 subunit of the cloned human gamma-aminobutyric acidA (GABAA) receptor (gamma 2 IL) was expressed in bacteria as glutathione-S-transferase and staphylococcal protein A fusion proteins. Mice were immunized with the fusion proteins (one protein per animal), and monoclonal antibodies were obtained. Six monoclonal antibodies reacted with the gamma 2 IL moiety of the fusion proteins. Three of these monoclonal antibodies also immunoprecipitated a high proportion of the GABAA/benzodiazepine receptors from bovine and rat brain and reacted with a wide 44,000-49,000-M(r) peptide band in immunoblots of affinity-purified GABAA receptors. These monoclonal antibodies are valuable reagents for the molecular characterization of the GABAA receptors in various brain regions.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-3042
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
64
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1305-11
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:7861163-Amino Acid Sequence,
pubmed-meshheading:7861163-Animals,
pubmed-meshheading:7861163-Antibodies, Monoclonal,
pubmed-meshheading:7861163-Antibody Specificity,
pubmed-meshheading:7861163-Base Sequence,
pubmed-meshheading:7861163-Cattle,
pubmed-meshheading:7861163-Cerebral Cortex,
pubmed-meshheading:7861163-DNA Primers,
pubmed-meshheading:7861163-Glutathione Transferase,
pubmed-meshheading:7861163-Humans,
pubmed-meshheading:7861163-Molecular Sequence Data,
pubmed-meshheading:7861163-Precipitin Tests,
pubmed-meshheading:7861163-Rats,
pubmed-meshheading:7861163-Receptors, GABA-A,
pubmed-meshheading:7861163-Recombinant Fusion Proteins,
pubmed-meshheading:7861163-Sequence Alignment,
pubmed-meshheading:7861163-Sequence Homology, Amino Acid
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pubmed:year |
1995
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pubmed:articleTitle |
Monoclonal antibodies to the human gamma 2 subunit of the GABAA/benzodiazepine receptors.
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pubmed:affiliation |
Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri-Kansas City 64110-2499.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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