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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
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pubmed:dateCreated |
1995-3-20
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pubmed:abstractText |
Retinal ganglion cells (RGCs) in rats were retrogradely labeled with the fluorescent tracer Fluorogold (FG) and subjected to GAP-43 and c-JUN immunocytochemistry to identify those RGCs that are capable of regenerating an axon. After optic nerve section (ONS) and simultaneous application of FG to the nerve stump (group 1 experiments), GAP-43 immunoreactive RGCs (between 2 and 21 days after ONS) always represented a subfraction of both FG-labeled (i.e., surviving) RGCs and RGCs exhibiting c-JUN. GAP-43 immunoreactive RGCs represented 22% of RGCs normally present in rat retinae and 25% of surviving RGCs at 5 days after ONS but were reduced to 2% and 1%, which is 6% and 5% of survivors at 14 and 21 days, respectively. In animals that received a peripheral nerve (PN) graft after ONS (group 2 experiments), RGCs with regenerating axons were identified by FG application to the graft at 14 and 21 days. When examined at 21 and 28 days, all FG-labeled RGCs exhibited GAP-43 immunoreactivity, and FG/GAP-43-labeled RGCs were 3% and 2% of those present in normal rat retinae. In relation to surviving RGCs GAP-43 immunoreactive RGCs represented 10% at both time points. FG-/GAP-43-labeled RGCs also exhibited c-JUN, but c-JUN immunoreactive RGCs were at both time points at least twice as numerous as FG-/GAP-43-labeled RGCs. These data suggest that regenerating axons in PN grafts derive specifically from GAP-43 reexpressing RGCs. Appearance of GAP-43 immunoreactivity may therefore identify those RGCs that are capable of axonal regeneration or sprouting.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-3034
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1570-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7861120-Animals,
pubmed-meshheading:7861120-Axons,
pubmed-meshheading:7861120-Female,
pubmed-meshheading:7861120-GAP-43 Protein,
pubmed-meshheading:7861120-Membrane Glycoproteins,
pubmed-meshheading:7861120-Nerve Regeneration,
pubmed-meshheading:7861120-Nerve Tissue Proteins,
pubmed-meshheading:7861120-Optic Nerve,
pubmed-meshheading:7861120-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:7861120-Rats,
pubmed-meshheading:7861120-Rats, Wistar,
pubmed-meshheading:7861120-Retinal Ganglion Cells,
pubmed-meshheading:7861120-Tissue Transplantation
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pubmed:year |
1994
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pubmed:articleTitle |
GAP-43 immunoreactivity and axon regeneration in retinal ganglion cells of the rat.
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pubmed:affiliation |
Faculty of Biology, University of Konstanz, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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