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rdf:type |
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lifeskim:mentions |
umls-concept:C0017082,
umls-concept:C0035647,
umls-concept:C0041365,
umls-concept:C0061099,
umls-concept:C0086418,
umls-concept:C0205088,
umls-concept:C0205195,
umls-concept:C0220781,
umls-concept:C0220806,
umls-concept:C0392756,
umls-concept:C1518422,
umls-concept:C1519249,
umls-concept:C1883254
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pubmed:issue |
7
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pubmed:dateCreated |
1995-3-20
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pubmed:abstractText |
N-Glycolylglucosamine 8 was synthesized in 4 steps from anisal glucosamine, via the new crystalline monochloracetyl derivatives 3, 4 and 7. N-Glycolylneuraminic acid 10 was prepared in 59% yield starting from pyruvate and a mixture of 8 and its manno epimer 9 in a 2:3 ratio, with immobilized sialic acid aldolase. Neu5Gc 10 was converted into CMP-NeuGc 11 in the presence of immobilized calf brain CMP-sialate synthetase. Finally 11 was used as a donor in the transfer to the acceptor beta-D-Gal-(1-3)-beta-D-GalNAc-OBn 12 catalyzed by a preparation of porcine liver (2-3)-alpha-sialyltransferase, roughly purified by a chromatography on Cibacron Blue-agarose. alpha-Neu5Gc-(2-3)-beta-D-Gal-(1-3)-beta-D-GalNac-OBn 13 isolated in 56% yield was deprotected to give the non-reducing terminal sequence of GM1b glycolylated ganglioside, which might be expressed in human tumors.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Enzymes, Immobilized,
http://linkedlifedata.com/resource/pubmed/chemical/Fructose-Bisphosphate Aldolase,
http://linkedlifedata.com/resource/pubmed/chemical/G(M1) Ganglioside,
http://linkedlifedata.com/resource/pubmed/chemical/Indicators and Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/N-Acylneuraminate...,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Sialyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Markers, Biological,
http://linkedlifedata.com/resource/pubmed/chemical/beta-galactoside...,
http://linkedlifedata.com/resource/pubmed/chemical/ganglioside M1b
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0968-0896
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
669-74
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:7858974-Animals,
pubmed-meshheading:7858974-Brain,
pubmed-meshheading:7858974-Carbohydrate Conformation,
pubmed-meshheading:7858974-Carbohydrate Sequence,
pubmed-meshheading:7858974-Cattle,
pubmed-meshheading:7858974-Chromatography, Affinity,
pubmed-meshheading:7858974-Enzymes, Immobilized,
pubmed-meshheading:7858974-Fructose-Bisphosphate Aldolase,
pubmed-meshheading:7858974-G(M1) Ganglioside,
pubmed-meshheading:7858974-Humans,
pubmed-meshheading:7858974-Indicators and Reagents,
pubmed-meshheading:7858974-Liver,
pubmed-meshheading:7858974-Magnetic Resonance Spectroscopy,
pubmed-meshheading:7858974-Molecular Sequence Data,
pubmed-meshheading:7858974-N-Acylneuraminate Cytidylyltransferase,
pubmed-meshheading:7858974-Neoplasms,
pubmed-meshheading:7858974-Oligopeptides,
pubmed-meshheading:7858974-Optical Rotation,
pubmed-meshheading:7858974-Sialyltransferases,
pubmed-meshheading:7858974-Swine,
pubmed-meshheading:7858974-Tumor Markers, Biological
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pubmed:year |
1994
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pubmed:articleTitle |
Combined chemical and enzymatic synthesis of the sialylated non reducing terminal sequence of GM1b glycolylated ganglioside, a potential human tumor marker.
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pubmed:affiliation |
Institut de Chimie Moléculaire d'Orsay, URA CNRS 462, Université Paris-Sud, Orsay, France.
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pubmed:publicationType |
Journal Article
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