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rdf:type |
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lifeskim:mentions |
umls-concept:C0001271,
umls-concept:C0001613,
umls-concept:C0007776,
umls-concept:C0015283,
umls-concept:C0022655,
umls-concept:C0205265,
umls-concept:C0337051,
umls-concept:C0376604,
umls-concept:C0456389,
umls-concept:C1509144,
umls-concept:C1521828,
umls-concept:C1555582,
umls-concept:C1622418,
umls-concept:C1882071,
umls-concept:C2587213
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pubmed:issue |
2
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pubmed:dateCreated |
1995-3-20
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pubmed:abstractText |
Morphological, biochemical, and membrane capacitance measurements were used to study the role of cortical filamentous actin (F-actin) in exocytosis. Fluorescence and electron microscopy of resting chromaffin cells revealed a cortical actin network that excluded secretory vesicles from the subplasmalemmal area. Phorbol ester (PMA) treatment disrupted cortical F-actin and increased both the number of vesicles within the 0-50 nm subplasmalemmal zone and the initial rate of stimulated catecholamine release. In PMA-pretreated cells, membrane capacitance studies showed an increased number of vesicles fusing with the plasmalemma during the first two depolarizations of a train. PMA did not affect voltage-dependent Ca2+ influx. The total number of vesicles fused with the plasma membrane correlated well with the number of vesicles occupying the 0-50 nm cortical zone. Therefore, cortical F-actin disassembly allows translocation of vesicles to the plasmalemma in preparation for exocytosis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0896-6273
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
353-63
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7857644-Actins,
pubmed-meshheading:7857644-Adrenal Cortex,
pubmed-meshheading:7857644-Animals,
pubmed-meshheading:7857644-Cattle,
pubmed-meshheading:7857644-Cell Membrane,
pubmed-meshheading:7857644-Cells, Cultured,
pubmed-meshheading:7857644-Chromaffin Granules,
pubmed-meshheading:7857644-Electrophysiology,
pubmed-meshheading:7857644-Epinephrine,
pubmed-meshheading:7857644-Exocytosis,
pubmed-meshheading:7857644-Kinetics,
pubmed-meshheading:7857644-Membrane Potentials,
pubmed-meshheading:7857644-Microscopy, Electron,
pubmed-meshheading:7857644-Nicotine,
pubmed-meshheading:7857644-Norepinephrine,
pubmed-meshheading:7857644-Potassium,
pubmed-meshheading:7857644-Tetradecanoylphorbol Acetate,
pubmed-meshheading:7857644-Video Recording
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pubmed:year |
1995
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pubmed:articleTitle |
Chromaffin cell cortical actin network dynamics control the size of the release-ready vesicle pool and the initial rate of exocytosis.
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pubmed:affiliation |
Department of Pharmacology, Faculty of Medicine, University of Ottawa, Ontario, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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