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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1995-3-14
|
| pubmed:abstractText |
The aim of this study is to investigate whether hepatitis B e antigen (HBeAg) reactivity can be detected on formalin-fixed, paraffin-embedded liver tissue, and whether immunohistochemical detection of intrahepatic HBeAg may help to distinguish between "wild-type" and "eminus" hepatitis B virus (HBV) infection. Liver biopsy specimens were analyzed from 27 patients with chronic type B hepatitis: 12 patients had serum HBeAg (group A), and 15 patients were anti-HBe positive (group B). Part of each biopsy fragment was processed for histologic and immunohistochemical studies, and a part was used for HBV-DNA analysis. Dewaxed sections from each specimen were tested with a specific monoclonal anti-HBe antibody; then a Biotin-Streptavidin kit was used as detection system. HBeAg was revealed in 10 of 12 cases of group A and in 6 of the 15 cases of group B. Pre-core region of HBV genomes, isolated from each biopsy specimen, was analyzed by direct sequencing: 10 cases of group A were found to be infected by wild-type HBV alone and 2 cases by both wild and e-minus HBV types. In group B, all the 6 cases with intrahepatic HBeAg reactivity were found to be infected by mixed viral population, whereas the 9 cases negative for such reactivity were found to be infected by e-minus HBV alone. These results show that HBeAg can be detected in formalin-fixed, paraffin-embedded liver specimens, and the method is sensitive and specific. Because the presence of HBeAg in the liver indicates a wild-type HBV infection, and the lack of detection of such antigen in the hepatocytes of anti-HBe positive subjects correlates with unmixed e-minus HBV infection, the authors conclude that this technique is a useful tool for recognizing the viral strains that infect patients with chronic type B hepatitis.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Fixatives,
http://linkedlifedata.com/resource/pubmed/chemical/Formaldehyde,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis B e Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotide Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Core Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0002-9173
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
103
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
136-40
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:7856554-Adolescent,
pubmed-meshheading:7856554-Adult,
pubmed-meshheading:7856554-Aged,
pubmed-meshheading:7856554-Base Sequence,
pubmed-meshheading:7856554-Biopsy,
pubmed-meshheading:7856554-Fixatives,
pubmed-meshheading:7856554-Formaldehyde,
pubmed-meshheading:7856554-Genetic Variation,
pubmed-meshheading:7856554-Genome, Viral,
pubmed-meshheading:7856554-Hepatitis B,
pubmed-meshheading:7856554-Hepatitis B e Antigens,
pubmed-meshheading:7856554-Hepatitis B virus,
pubmed-meshheading:7856554-Humans,
pubmed-meshheading:7856554-Liver,
pubmed-meshheading:7856554-Middle Aged,
pubmed-meshheading:7856554-Molecular Sequence Data,
pubmed-meshheading:7856554-Oligonucleotide Probes,
pubmed-meshheading:7856554-Polymerase Chain Reaction,
pubmed-meshheading:7856554-Viral Core Proteins
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Hepatitis B E antigen detection in formalin-fixed liver biopsy specimens. A tool to investigate wild-type and E-minus variant HBV infection.
|
| pubmed:affiliation |
Dipartimento di Patologia Umana, Università di Messina, Italy.
|
| pubmed:publicationType |
Journal Article
|